[Histonet] "GOLD" slides??
gcallis <@t> montana.edu
Fri Jan 5 11:08:38 CST 2007
Have always been under the impression these are for use with frozen
sections as the package insert states (quote):
1. Inherently tissue binding so there is no need for sprays or adhesives
with these slides.
2. Allow plus gold slides to contact the forzen sections directly in
cryostat using standard technic
3. Place slide horizontally on table top at room temp for 2- 30 seconda
for fresh tissue (frozens) or 1 minutes for fixed
frozen sections. There was more info here, but the longer the bonding
time, the better the frozen sections adhere to slides.
4. Postfix in acetone or alcohol based fixative, postfixation in NBF is
not recommended. Stain per a standard protocol.
I am not so sure that "nothing" falls off these slides. They are far more
expensive than the regular Plus charge, the boxes we have had only 25
slides. These little gold nuggets were approx double the cost of regular
plus charge, We have used them with problematic frozen sections, but not
for for formalin fixed paraffin embedded (FFPE) sections. There are people
who use them for FFPE sections, swear by their holding capabilities - there
are replies on Histonet about using them. The only time we can justify the
cost is with a really problematic frozen section release and if that is a
terrible problem, we go to the Cryojane where the sections do NOT release
from the surface.
Erie Scientific also developed another coated slide, called Extra for use
with EDTA antigen retrieval. Apparently high pH EDTA raises havoc with a
glass slide surface, basically chelates ions from the surface, leading to
release of sections. If this is a problem you experience you should
contact Erie Scientific for specifics.
Our fisher cat. no. was 22-035813, which may have changed since we bought
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-4303 (FAX)
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