[Histonet] GRAM Stain Timing Question

Blazek, Linda lblazek <@t> digestivespecialists.com
Wed Feb 28 13:47:58 CST 2007

Can't your legacy be "differentiate until it looks right"?  If I recall
right (and that is a question in it's self) when the color stops running
off the slide the differentiation is complete.  To go any further you
will decolorize the gram-positive thingy bobs.

Linda Blazek HT (ASCP)
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lblazek <@t> digestivespecialists.com


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Breeden,
Sent: Wednesday, February 28, 2007 2:16 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] GRAM Stain Timing Question

I seem to do a lot of GRAM stains and although I've modified some of the
times to suit my pathologists' tastes, I have never been able to pin
down the first differentiation time in 100% alcohol.  Today, I'm using
10 quick dips although it's only because the wind is blowing and the
moon is on the rise.  Can someone give me a more specific guideline for
differentiating after the Gram's Iodine and before the Safranin? I'd
like to leave a more definite legacy when I retire than "differentiate
in 100% alcohol or acetone"...  I'm thankin' you in advance.  And I
think we all know who the Purple Haze Generation are on THIS media,
don't we???


Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 700

Albuquerque, NM  87106



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