[Histonet] Questions for John Kiernan
hawkmoon15 <@t> cox.net
Mon Feb 26 15:36:14 CST 2007
I have a couple of questions for you, if you would be so kind as to answer:
1) Is there a problem with the Alcian Blue that's on the market today? We
are having problems with the acid mucin being masked by the hematoxylin. I
am aware that some hematoxylins do stain mucin, but this just doesn't look
like the AB/PAS-H I have been used to seeing over the years.
2) Is there a treatment for aspergillus that will cause it not to stain
with Schiff's? I have used aspergillus for a PAS fungus control for years,
and have never seen this problem before. It stains fine with a GMS.
Thank you for any help you can give me. I have your book Histological and
Histochemical Methods Theory & Practice and have not found the answer to my
fungus question in it, although you explain about Alcian Blue and I found
reading that helpful.
Thank you again!
Sarah A. Jones
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