[Histonet] staining problems
staceylburton <@t> yahoo.com
Mon Feb 26 13:27:40 CST 2007
I agree with the advise given so far. Your problem is most likely in the "deparaffinization" process. Try changing out and using fresh xylene - maybe bump the time up a little also by one - two minutes. * Are you using a xylene substitute? If so, this could also be your problem - try changing back to good old xylene for paraffin removal on your slides for IHC staining - 4 changes @ 3 - minutes each.
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