[Histonet] staining problems

Stacey Burton staceylburton <@t> yahoo.com
Mon Feb 26 13:27:40 CST 2007

I agree with the advise given so far. Your problem is most likely in the "deparaffinization" process.  Try changing out and using fresh xylene - maybe bump the time up a little also by one - two minutes.  * Are you using a xylene substitute?  If so, this could also be your problem - try changing back to good old xylene for paraffin removal on your slides for IHC staining - 4 changes @ 3 - minutes each.  

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