[Histonet] Alkaline Phosphatase.

Ian Montgomery ian.montgomery <@t> bio.gla.ac.uk
Fri Feb 23 10:20:50 CST 2007

    Several weeks ago there was a request for an alkaline phosphatase
technique that could be used on wax sections. My humble apologies but I
overlooked replying.
    My method of choice is, McGadey,J. 1970. Histochemie. 23. 180-184.
Tetrazolium method for non-specific Alkaline Phosphatase.
    I've used the technique on cryostat sections, fresh or lightly fixed in
Baker's formol calcium, carefully processed paraffin sections and resin
embedded sections. In all instances the method worked superbly. If you're
interested and cannot get the journal let me know and I'll e-mail the

Dr. Ian Montgomery,
IBLS Support Services,
Thomson Building,
University of Glasgow,
Extn: 8511.

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