[Histonet] Alkaline Phosphatase.

[Ian Montgomery]

    Several weeks ago there was a request for an alkaline phosphatase
technique that could be used on wax sections. My humble apologies but I
overlooked replying.
    My method of choice is, McGadey,J. 1970. Histochemie. 23. 180-184.
Tetrazolium method for non-specific Alkaline Phosphatase.
    I've used the technique on cryostat sections, fresh or lightly fixed in
Baker's formol calcium, carefully processed paraffin sections and resin
embedded sections. In all instances the method worked superbly. If you're
interested and cannot get the journal let me know and I'll e-mail the
technique.
Ian.


Dr. Ian Montgomery,
Histotechnology,
IBLS Support Services,
Thomson Building,
University of Glasgow,
Tel:01413398855
Extn: 8511.