[Histonet] toenails

Hopkins, Karen KMH.02 <@t> ex.uchs.org
Fri Feb 23 07:26:03 CST 2007 

We used to cut toenails by the hundreds and all we ever did was process on a routine processing cycle then embed as usual but on edge horizontally in the cassette.  We put albumin on our slides to hold the nail on and stained them routinely.  They were no problem once you got used to them.

Also, I agree with Hector concerning microwaves- I do as he suggested.

Karen Hopkins
Histology Supervisor
Upper Chesapeake Medical Center & 
Harford Memorial Hospital

p. 443-643-1454
f. 443-643-1450

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, February 23, 2007 6:26 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 39, Issue 35

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Today's Topics:

   1. Re: cryostat (rsrichmond <@t> aol.com)
   2. PAS stain for nail fungus (rsrichmond <@t> aol.com)
   3. Stain for cerebral blood vessels (Laura Harris)
   4. IHC ER/PR controls (Joyce Cline)
   5. RE: IHC ER/PR controls (Sebree Linda A.)
   6. AW: [Histonet] Stain for cerebral blood vessels (Gudrun Lang)
   7. RE: New Microwave Cap Regulation (Luck, Greg D.)
   8. New Microwave Cap Regulation (Stacey Burton)
   9. RE: New Microwave Cap Regulation (Rene J Buesa)
  10. Dulbecco's PBS (garciaa <@t> mskcc.org)
  11. Re: FDA approved recyclers (Vilma Kvieskaite)
  12. Re: FDA approved recyclers (Rene J Buesa)
  13. FW: Microwave use (Bonnie Whitaker)
  14. unsubscribe (Theleria Hackett)
  15. RE: New Microwave Cap Regulation (Hector Hernandez)
  16. Re: PAS stain for nail fungus (Joe Nocito)
  17. LIS programs (Marilyn McDonald)
  18. Re: PAS stain for nail fungus (Akemi Allison-Tacha)
  19. I have an E300 (M C)
  20. RE: LIS programs (Bonnie Whitaker)
  21. Re: PAS stain for nail fungus (Joe Nocito)
  22. RE: FDA approved recyclers (Lee & Peggy Wenk)
  23. RE: LIS programs (Weems, Joyce)


----------------------------------------------------------------------

Message: 1
Date: Thu, 22 Feb 2007 13:02:43 -0500
From: rsrichmond <@t> aol.com
Subject: [Histonet] Re: cryostat
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8C924C2C8E4DABE-1784-3195 <@t> MBLK-M22.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

Jane Moose at Newberry County Memorial Hospital in Newberry, SC notes that she's been approved for a new cryostat.
 
This reminds me of a reason to replace old cryostats - they use the refrigerant gas R-12, which is no longer legal in the USA (though still available) because it's a prohibited fluorocarbon. The information as to what refrigerant is used is on the base plate of the cryostat.
 
Bob Richmond
Samurai Pathologist
Knoxville TN
(alas - no SC medical license)
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Message: 2
Date: Thu, 22 Feb 2007 13:09:24 -0500
From: rsrichmond <@t> aol.com
Subject: [Histonet] PAS stain for nail fungus
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8C924C3B79DE816-1784-31C9 <@t> MBLK-M22.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

Dr. Allen Smith's recent post reminds me of a question I've been meaning to post to Histonet.
 
Podiatrists are now performing biopsies of toenails with nail fungus, in order to get a fungal stain (PAS is being requested, I think) done. Apparently the insurance companies are now requiring a biopsy or culture diagnosis of fungal disease before they'll pay for the very expensive systemic drugs for nail fungus like terbinafine (Lamisil). 
 
Is anyone on Histonet getting these requests? What protocols do you use to cut and stain these difficult specimens?
 
Bob Richmond
Samurai Pathologist
Knoxville TN
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Message: 3
Date: Thu, 22 Feb 2007 18:13:18 +0000
From: Laura Harris <ljwh2 <@t> cam.ac.uk>
Subject: [Histonet] Stain for cerebral blood vessels
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <45DDDD3E.4000708 <@t> cam.ac.uk>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Dear Histonet,

I'm a molecular biologist working at the University of Cambridge, UK. I
was wondering if anyone could recommend a histochemical stain that would
work for cerebral blood vessels, preferably in frozen sections? I've
been trying alkaline phosphatase staining but with mixed results.
Would a collagen stain like Fast Green FGF work in isolation (rather
than as part of a trichrome stain)?

It doesn't need to be completely specific for vessels, as long as they
are clearly visible. The other point is that is needs to be as quick and
simple as possible, as I need to extract protein from the tissue
afterwards. Immunostaining is no good for this application. All 
suggestions welcome as I will probably need to test several things to 
find one that works.

Best wishes, Laura Harris






------------------------------

Message: 4
Date: Thu, 22 Feb 2007 13:22:56 -0500
From: "Joyce Cline" <jcline <@t> wchsys.org>
Subject: [Histonet] IHC ER/PR controls
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000401c756ae$79576f60$1d2a14ac <@t> wchsys.org>
Content-Type: text/plain;charset="us-ascii"

This question is for anyone who uses a Benchmark XT.  We would like to
know at what percentages your pathologists prefer their controls. 11-25%
weak staining or do they prefer the ER/PR controls with a stronger
percentage?


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Message: 5
Date: Thu, 22 Feb 2007 12:35:47 -0600
From: "Sebree Linda A." <LSebree <@t> uwhealth.org>
Subject: RE: [Histonet] IHC ER/PR controls
To: "Joyce Cline" <jcline <@t> wchsys.org>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<E356FBF208CDC844AA47BF2339653D799D8835 <@t> uwhis-xchng4.uwhis.hosp.wisc.edu>
	
Content-Type: text/plain;	charset="us-ascii"

We usually go with stronger controls (scores of 12 out of 12 or slightly
lower).  This probably isn't the best; an array of intensities would be
but no one is complaining.

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joyce
Cline
Sent: Thursday, February 22, 2007 12:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC ER/PR controls


This question is for anyone who uses a Benchmark XT.  We would like to
know at what percentages your pathologists prefer their controls. 11-25%
weak staining or do they prefer the ER/PR controls with a stronger
percentage?


***** CONFIDENTIALITY NOTICE *****
This message contains confidential information and is intended only for
the individual named. If you are not the named addressee you should not
disseminate, distribute or copy this e-mail. Please notify the sender
immediately by e-mail if you have received this e-mail by mistake and
delete this e-mail from your system.

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------------------------------

Message: 6
Date: Thu, 22 Feb 2007 19:54:53 +0100
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] Stain for cerebral blood vessels
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001601c756b2$f25f5590$6412a8c0 <@t> dielangs.at>
Content-Type: text/plain;	charset="iso-8859-1"

I think vanGieson is the shortest collagen-stain. Staining with FGF or
Anilinblue alone would perhaps lead to an overall stain, more in the
collagen fibers less in the rest of the tissue.

These are links to the excellent pathopic-picture-archive. Perhaps you find
the right stain for your needs.
HE: http://alf3.urz.unibas.ch/pathopic/getpic-fra.cfm?id=006513
PAS: http://alf3.urz.unibas.ch/pathopic/getpic-fra.cfm?id=006507


Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Laura
Harris
Gesendet: Donnerstag, 22. Februar 2007 19:13
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Stain for cerebral blood vessels

Dear Histonet,

I'm a molecular biologist working at the University of Cambridge, UK. I
was wondering if anyone could recommend a histochemical stain that would
work for cerebral blood vessels, preferably in frozen sections? I've
been trying alkaline phosphatase staining but with mixed results.
Would a collagen stain like Fast Green FGF work in isolation (rather
than as part of a trichrome stain)?

It doesn't need to be completely specific for vessels, as long as they
are clearly visible. The other point is that is needs to be as quick and
simple as possible, as I need to extract protein from the tissue
afterwards. Immunostaining is no good for this application. All 
suggestions welcome as I will probably need to test several things to 
find one that works.

Best wishes, Laura Harris




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Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 7
Date: Thu, 22 Feb 2007 11:33:16 -0800
From: "Luck, Greg D." <LuckG <@t> empirehealth.org>
Subject: RE: [Histonet] New Microwave Cap Regulation
To: "Stacey Burton" <staceylburton <@t> yahoo.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<6BB8BC4519AAB844B174FC739A679BBCCEFCC5 <@t> IRMEXCH01.irm.inhs.org>
Content-Type: text/plain;	charset="us-ascii"

Hello all,
Would venting or use of a "Laboratory Grade" microwave be required if
all that it is used for is to heat water or PBS or Tris buffers?
Thanks, Greg
Greg Luck, B.S., HT(ASCP)
Anatomic Pathology Supervisor
Deaconess Med Cntr
800 W. 5th Ave
Spokane, WA 99204
Offc 509.473.7077
Fax 509.473.7133
luckg <@t> empirehealth.org  
www.deaconessmc.org
 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stacey
Burton
Sent: Thursday, February 22, 2007 8:22 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] New Microwave Cap Regulation

I am curious to know how labs have responding to the CAP Question
ANT.29430 which asks "Are microwave devices properly vented?".
Have the labs continued to utilized their regular "kitchen model"
microwaves and applied duct work to them to be vented out of the
building?
Please comment.
 
Thank you,
Stacey Burton, H.T. ASCP
Precision Pathology Services
San Antonio Texas


 
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Message: 8
Date: Thu, 22 Feb 2007 12:08:20 -0800 (PST)
From: Stacey Burton <staceylburton <@t> yahoo.com>
Subject: [Histonet] New Microwave Cap Regulation
To: Histonet Listserver <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <537399.57004.qm <@t> web30003.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

----- Forwarded Message ----
From: Bonnie Whitaker <bwhitaker <@t> brownpathology.com>
To: Stacey Burton <staceylburton <@t> yahoo.com>
Sent: Thursday, February 22, 2007 1:45:13 PM
Subject: RE: [Histonet] New Microwave Cap Regulation


Hi Stacey,

My understanding is that this is N/A unless you are processing in your
microwave, or using other "dangerous" stuff.  My lab is considering this not
applicable because we only use the microwave to heat water, and a few
stains.  We don't heat Bouins, or any other fixative, dehydrant or clearing
agents.  I can let you know how if this is acceptable after our inspection
that could be any time from now until May.

Bonnie Whitaker


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stacey
Burton
Sent: Thursday, February 22, 2007 10:22 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] New Microwave Cap Regulation


I am curious to know how labs have responding to the CAP Question  ANT.29430
which asks "Are microwave devices properly vented?". Have the labs continued
to utilized their regular "kitchen model" microwaves and applied duct work
to them to be vented out of the building? Please comment.

Thank you,
Stacey Burton, H.T. ASCP
Precision Pathology Services
San Antonio Texas



____________________________________________________________________________
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------------------------------

Message: 9
Date: Thu, 22 Feb 2007 12:18:36 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: RE: [Histonet] New Microwave Cap Regulation
To: "Luck, Greg D." <LuckG <@t> empirehealth.org>,	Stacey Burton
	<staceylburton <@t> yahoo.com>, histonet <@t> lists.utsouthwestern.edu
Message-ID: <439567.55282.qm <@t> web61214.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Greg:
  The new CAP regulation stems from the fact that many labs used the "house microwaves ovens"(HMWO) also to fix and process tissues. That is where CAP, manufacturers and a MW task force intervened with the valid warning that fixing and processing, and even staining when the solutions are alcoholic, are unsafe procedures to be done in a regular
  HMWO,and that those procedures really require an instrument with a venting capability that will take the noxious vapors out of the lab.
  To just heat water or buffers for HIER I did that for many years and no inspector ever said anything. Perhaps they have become more demanding recently on this regulation, but for me there is nothing noxious to vent when only water, PBS or buffers are heated. In your house you also heat water and there is nothing noxious about it (and we are talking about the "sacred" home environment).
  Just my opinion though!
  René J.

"Luck, Greg D." <LuckG <@t> empirehealth.org> wrote:
  Hello all,
Would venting or use of a "Laboratory Grade" microwave be required if
all that it is used for is to heat water or PBS or Tris buffers?
Thanks, Greg
Greg Luck, B.S., HT(ASCP)
Anatomic Pathology Supervisor
Deaconess Med Cntr
800 W. 5th Ave
Spokane, WA 99204
Offc 509.473.7077
Fax 509.473.7133
luckg <@t> empirehealth.org 
www.deaconessmc.org



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stacey
Burton
Sent: Thursday, February 22, 2007 8:22 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] New Microwave Cap Regulation

I am curious to know how labs have responding to the CAP Question
ANT.29430 which asks "Are microwave devices properly vented?".
Have the labs continued to utilized their regular "kitchen model"
microwaves and applied duct work to them to be vented out of the
building?
Please comment.

Thank you,
Stacey Burton, H.T. ASCP
Precision Pathology Services
San Antonio Texas



________________________________________________________________________
____________
Food fight? Enjoy some healthy debate
in the Yahoo! Answers Food & Drink Q&A.
http://answers.yahoo.com/dir/?link=list&sid=396545367
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Message: 10
Date: Thu, 22 Feb 2007 15:25:06 -0500
From: garciaa <@t> mskcc.org
Subject: [Histonet] Dulbecco's PBS
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<597B9BC80B36084781D3951A88205F12316878 <@t> SMSKPEXMBX8.MSKCC.ROOT.MSKCC.ORG>
	
Content-Type: text/plain; charset=iso-8859-1

Hi, everyone.  I have a few questions I'm hoping you'll be able to help me out with.  I have 10+ years doing immunohistochemistry on PFA perfused, frozen brain sections, but a few things I'd like clarified.  

1)  Does it matter whether one uses PBS with or without Mg and Ca for immunos?  I have always done it with PBS without Mg and Ca, and have never heard of anyone doing it with Mg and Ca, but have recently encountered a scenario in which this was the only PBS available.  Wondering whether this will make a difference in my staining.

2)  Can anyone explain the need for dehydrating and defatting slides (after using such substrates as DAB for color development) before coverslipping?  Aside from the obvious that the sections look pretty crappy without these steps, can anyone explain the science behind this?  

3)  Because my tissue is perfused, I typically store the brains in 30% sucrose (with a little bit of sodium azide) in 4 degrees, until I am ready to cut the blocks.  At this point, brains are usually frozen in the cryostat just before cutting 40um thick sections.  I have obtained wonderful staining and morphology with this technique, however I have been recently advised to store the brains embedded in OCT (or similar) in a -20 degree freezer, rather than in 4 degrees.  I am skeptical and concerned that this will damage the morphology and produce that "swiss cheese" freezer artifact (you know, where there are all these holes in the tissue?)  Can anyone provide any advice, insight as to the benefits and drawbacks to either/both of these methods?

Thanks everyone!  Any advice, insight, comments are greatly appreciated.

D. Garcia

 
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Message: 11
Date: Thu, 22 Feb 2007 12:25:55 -0800 (PST)
From: Vilma Kvieskaite <kvieskaite <@t> yahoo.com>
Subject: Re: [Histonet] FDA approved recyclers
To: histonet <@t> lists.utsouthwestern.edu
Cc: tmmrosla <@t> healtheast.org
Message-ID: <20070222202555.55831.qmail <@t> web38502.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hallo,
   
  Maybe do you have some time to answer few questions? Do you use B/R Procycler 9700 for buffered formalin recycling? It is worth? Do you have some problems with this equipment? Do you filtrate formalin before recycling? What filters do you use? How do you make buffered formalin after recycling- maybe do you have special enclosed system for buffer preparation? 
   
  Thanks, 
  Vilma
  

"Mrosla, Tina M" <tmmrosla <@t> healtheast.org> wrote:
  
We were just informed that some hospitals don't recycle xylene, alcohol, or formalin because solvent recyclers are not approved by the FDA for use in hospitals. Is this true? 
We have a B/R Procycler 9700 that we have been using for 5 years. Does anyone know anything about this recycler and FDA approval?

Tina Mrosla, HTL (ASCP)
Histotechnologist
St. Joseph's Hospital
Healtheast Care System
651-232-3575
fax 232-4543
tmmrosla <@t> healtheast.org

Passion for Caring and Service



The information included in this e-mail message, including any attachments, is intended only for the person or organization to which it is addressed. This e-mail message may contain information that is privileged or confidential. If you receive this e-mail message and are not the intended recipient or responsible for delivering the message to the intended recipient, you may not use, disseminate, distribute or copy the information included in this e-mail and any attachments. If you received this e-mail message by mistake, please reply by e-mail and destroy all copies of this message and any attachments. Thank you.

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Message: 12
Date: Thu, 22 Feb 2007 12:47:42 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] FDA approved recyclers
To: Vilma Kvieskaite <kvieskaite <@t> yahoo.com>,
	histonet <@t> lists.utsouthwestern.edu
Cc: tmmrosla <@t> healtheast.org
Message-ID: <938619.58926.qm <@t> web61211.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Vilma:
  I never recycled formalin and let me tell you why:
  in the first place you BUY the neutral (buffered) formalin to avoid exposing yourself and staff to formalin.
  If you recycle it you defeat the whole initial purpose because at the end you will end with formalin that YOU will have to dilute, that YOU will have to neutralize and YOU will end doing what you wanted to avoid in the first place.
  Although it is expensive to dispose of formalin (via a specialized company) it is worth it!
  At least that is how I saw the whole issue and decided NOT to recycle formalin.
   
  With regards to ethanol I did not recycle it either because it takes TWICE the amount of time to recycle ethanol than to recycle xylene and is cheaper to dilute it and dispose it to the sewer system.
   
  I only recycled xylene and my B/R recycled was paid off in juts 34 months.
  René J.

Vilma Kvieskaite <kvieskaite <@t> yahoo.com> wrote:
  Hallo,

Maybe do you have some time to answer few questions? Do you use B/R Procycler 9700 for buffered formalin recycling? It is worth? Do you have some problems with this equipment? Do you filtrate formalin before recycling? What filters do you use? How do you make buffered formalin after recycling- maybe do you have special enclosed system for buffer preparation? 

Thanks, 
Vilma


"Mrosla, Tina M" wrote:

We were just informed that some hospitals don't recycle xylene, alcohol, or formalin because solvent recyclers are not approved by the FDA for use in hospitals. Is this true? 
We have a B/R Procycler 9700 that we have been using for 5 years. Does anyone know anything about this recycler and FDA approval?

Tina Mrosla, HTL (ASCP)
Histotechnologist
St. Joseph's Hospital
Healtheast Care System
651-232-3575
fax 232-4543
tmmrosla <@t> healtheast.org

Passion for Caring and Service



The information included in this e-mail message, including any attachments, is intended only for the person or organization to which it is addressed. This e-mail message may contain information that is privileged or confidential. If you receive this e-mail message and are not the intended recipient or responsible for delivering the message to the intended recipient, you may not use, disseminate, distribute or copy the information included in this e-mail and any attachments. If you received this e-mail message by mistake, please reply by e-mail and destroy all copies of this message and any attachments. Thank you.

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Message: 13
Date: Thu, 22 Feb 2007 15:56:21 -0600
From: "Bonnie Whitaker" <bwhitaker <@t> brownpathology.com>
Subject: [Histonet] FW: Microwave use
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: staceylburton <@t> yahoo.com
Message-ID: <000a01c756cc$4993d250$3601a8c0 <@t> brownpathology.net>
Content-Type: text/plain;	charset="us-ascii"

Well, it looks like I am wrong about being able to use my microwave to heat
water, buffer, etc.... at least I checked and the Instruction Booklet does
say "not for laboratory use" in the Safety Instructions portion.  See a
vendor's private message to me below (thanks, Donna).
 
Bonnie Whitaker
-----Original Message-----
From: Donna Willis [mailto:donna <@t> milestonemed.com] 
Sent: Thursday, February 22, 2007 2:52 PM
To: 'Bonnie Whitaker'
Subject: Microwave use



Bonnie,

I decided to email you direct on this issue.  Your onsite inspection will be
with the 2005 CAP regulations.  They have changed as of Dec 2006 and your
next year self inspection will be with the new regs.  The regs say that you
have to use the microwave according to manufacture guidelines.  Household
units have in their warranty that they are for food processing, do not use
for laboratory use.  This has always been an issue with OSHA but never
mentioned in CAP.  CAP has changed and added the new question. OSHA 29 CFR
1910.303(b)(2)  (listed or labeled equipment shall be used or installed in
accordance with any instructions included in the listing or labeling)  has
always been an issue but rarely enforced.  As far as OSHA is concerned you
should never use any piece of equipment out side of the manufacture listing.
This would mean no rice steamers in the lab for AR.  I have put the new regs
in my workshop.  I'll let you know the reaction of folks as I give them this
year.

 

Being a vendor I felt it would be better to just e-mail you myself instead
of everyone on the histonet.  If you feel you want to post this to the net,
it is up to you.

 

 

Donna Willis,HT(ASCP)HTL

Milestone Medical

North American Application Manager

2100 N. Hwy 360 Suite 506

Grand Prairie, Tx  75050

972-606-9986 office

214-725-6184 cell

 



------------------------------

Message: 14
Date: Thu, 22 Feb 2007 17:15:22 -0500
From: "Theleria Hackett" <thackett <@t> ils-inc.com>
Subject: [Histonet] unsubscribe
To: <HistoNet <@t> pathology.swmed.edu>
Message-ID:
	<8C8BF3DF11143944BFD681B6FD64A51A051BDB <@t> ILSExchange.ILS.Mail>
Content-Type: text/plain;	charset="iso-8859-1"

 
 
Theleria R. Hackett, B.S., HT (ASCP)CM 
Program Manager, Histology
Comparative Molecular Pathology Division
ILS, Inc. 
P.O. Box 13501 
Research Triangle Park, NC 27709 
(919) 281-1110 ext. 730 
(919) 281-1118 Fax 
thackett <@t> ils-inc.com <mailto:thackett <@t> ils-inc.com>  
www.ils-inc.com <http://www.ils-inc.com/>  
 

  
  
THIS MESSAGE IS INTENDED ONLY FOR THE USE OF THE PARTY TO WHOM IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL, AND PROTECTED FROM DISCLOSURE UNDER LAW. If you are not the addressee, or a person authorized to deliver the document to the addressee, you are hereby notified that any review, disclosure, dissemination, copying, or other action based on the content of this communication is not authorized. If you have received this document in error, please immediately notify us by email or telephone.
 


------------------------------

Message: 15
Date: Thu, 22 Feb 2007 16:19:54 -0600
From: "Hector Hernandez" <hhernandez <@t> pathreflab.com>
Subject: RE: [Histonet] New Microwave Cap Regulation
To: "'Rene J Buesa'" <rjbuesa <@t> yahoo.com>,	"'Luck, Greg D.'"
	<LuckG <@t> empirehealth.org>,	"'Stacey Burton'" <staceylburton <@t> yahoo.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<mailman.708.1172229890.25325.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain;	charset="iso-8859-1"

ANP.29430   Phase 1
This question doesn't state laboratory or home grade microwaves.  Microwave
should be placed in an appropriate ventilated hood to contain airborne
chemical contaminates and potential infectious agents. Microwaves used
outside a fume hood should have an integral fume extractor that is certified
by the manufacturer for use in a clinical laboratory.  This doesn't say
microwave has to be laboratory grade, only the fume extractor. This
checklist question doesn't apply if only non-hazardous reagents are used in
the device (microwave) (e.g. water, certain biological stains)  
 
In my opinion you can use any microwave (laboratory or house microwave) as
long as you can document:
1. Leakage is less than 5mW/cm2 at a distance of 5 cm from the surface.
2. Periodically monitor for temperature reproducibility.
3. All containers used in the microwave are made from microwave-transparent
material/ microwave proof.
Don't make it any harder than it is.

Hope this clears a few things,

Hector


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, February 22, 2007 2:19 PM
To: Luck, Greg D.; Stacey Burton; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] New Microwave Cap Regulation

Greg:
  The new CAP regulation stems from the fact that many labs used the "house
microwaves ovens"(HMWO) also to fix and process tissues. That is where CAP,
manufacturers and a MW task force intervened with the valid warning that
fixing and processing, and even staining when the solutions are alcoholic,
are unsafe procedures to be done in a regular
  HMWO,and that those procedures really require an instrument with a venting
capability that will take the noxious vapors out of the lab.
  To just heat water or buffers for HIER I did that for many years and no
inspector ever said anything. Perhaps they have become more demanding
recently on this regulation, but for me there is nothing noxious to vent
when only water, PBS or buffers are heated. In your house you also heat
water and there is nothing noxious about it (and we are talking about the
"sacred" home environment).
  Just my opinion though!
  René J.

"Luck, Greg D." <LuckG <@t> empirehealth.org> wrote:
  Hello all,
Would venting or use of a "Laboratory Grade" microwave be required if
all that it is used for is to heat water or PBS or Tris buffers?
Thanks, Greg
Greg Luck, B.S., HT(ASCP)
Anatomic Pathology Supervisor
Deaconess Med Cntr
800 W. 5th Ave
Spokane, WA 99204
Offc 509.473.7077
Fax 509.473.7133
luckg <@t> empirehealth.org 
www.deaconessmc.org



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stacey
Burton
Sent: Thursday, February 22, 2007 8:22 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] New Microwave Cap Regulation

I am curious to know how labs have responding to the CAP Question
ANT.29430 which asks "Are microwave devices properly vented?".
Have the labs continued to utilized their regular "kitchen model"
microwaves and applied duct work to them to be vented out of the
building?
Please comment.

Thank you,
Stacey Burton, H.T. ASCP
Precision Pathology Services
San Antonio Texas



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in the Yahoo! Answers Food & Drink Q&A.
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------------------------------

Message: 16
Date: Thu, 22 Feb 2007 17:03:08 -0600
From: "Joe Nocito" <jnocito <@t> satx.rr.com>
Subject: Re: [Histonet] PAS stain for nail fungus
To: <rsrichmond <@t> aol.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <00c901c756d5$9e5609d0$649eae18 <@t> yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

Hey Doc,
you have the South Texas Toenail lab guy right here. That's why I'm known as 
"Joe the Toe".
    We soak our nails in 20% ammonium hydroxide for at least an hour before 
we put the blocks on the machine to process. We've been getting nailed for a 
couple of years and perform a PAS for Fungus on each case that we do. 
Reimbursement must be good, we keep getting nailed.

Joe the Toe or just JTT

----- Original Message ----- 
From: <rsrichmond <@t> aol.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, February 22, 2007 12:09 PM
Subject: [Histonet] PAS stain for nail fungus


> Dr. Allen Smith's recent post reminds me of a question I've been meaning 
> to post to Histonet.
>
> Podiatrists are now performing biopsies of toenails with nail fungus, in 
> order to get a fungal stain (PAS is being requested, I think) done. 
> Apparently the insurance companies are now requiring a biopsy or culture 
> diagnosis of fungal disease before they'll pay for the very expensive 
> systemic drugs for nail fungus like terbinafine (Lamisil).
>
> Is anyone on Histonet getting these requests? What protocols do you use to 
> cut and stain these difficult specimens?
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
> ________________________________________________________________________
> Check out the new AOL.  Most comprehensive set of free safety and security 
> tools, free access to millions of high-quality videos from across the web, 
> free AOL Mail and more.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------

Message: 17
Date: Thu, 22 Feb 2007 17:47:21 -0600
From: Marilyn McDonald <kildee6093 <@t> mac.com>
Subject: [Histonet] LIS programs
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <2E9C4E57-F430-4180-AF78-43EE869A89B5 <@t> mac.com>
Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed

What is your favorite Pathology program? What do you like about it?   
I am familiar with Tamtron (Powerpath).  A friend has asked me so I  
decided to post it on the Histonet.  No vendors please.
Thanks




------------------------------

Message: 18
Date: Thu, 22 Feb 2007 15:51:19 -0800 (PST)
From: Akemi Allison-Tacha <akemiat3377 <@t> yahoo.com>
Subject: Re: [Histonet] PAS stain for nail fungus
To: Joe Nocito <jnocito <@t> satx.rr.com>, rsrichmond <@t> aol.com,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <86477.62101.qm <@t> web31309.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi Joe,

I guess the histology field still uses the old
methods.  I was taught that back in 1974 at OHSU. 
FYI-We also used soak in NAIR HAIR REMOVAL to soften
nails. 
Akemi Allison-Tacha 


--- Joe Nocito <jnocito <@t> satx.rr.com> wrote:

> Hey Doc,
> you have the South Texas Toenail lab guy right here.
> That's why I'm known as 
> "Joe the Toe".
>     We soak our nails in 20% ammonium hydroxide for
> at least an hour before 
> we put the blocks on the machine to process. We've
> been getting nailed for a 
> couple of years and perform a PAS for Fungus on each
> case that we do. 
> Reimbursement must be good, we keep getting nailed.
> 
> Joe the Toe or just JTT
> 
> ----- Original Message ----- 
> From: <rsrichmond <@t> aol.com>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Thursday, February 22, 2007 12:09 PM
> Subject: [Histonet] PAS stain for nail fungus
> 
> 
> > Dr. Allen Smith's recent post reminds me of a
> question I've been meaning 
> > to post to Histonet.
> >
> > Podiatrists are now performing biopsies of
> toenails with nail fungus, in 
> > order to get a fungal stain (PAS is being
> requested, I think) done. 
> > Apparently the insurance companies are now
> requiring a biopsy or culture 
> > diagnosis of fungal disease before they'll pay for
> the very expensive 
> > systemic drugs for nail fungus like terbinafine
> (Lamisil).
> >
> > Is anyone on Histonet getting these requests? What
> protocols do you use to 
> > cut and stain these difficult specimens?
> >
> > Bob Richmond
> > Samurai Pathologist
> > Knoxville TN
> >
>
________________________________________________________________________
> > Check out the new AOL.  Most comprehensive set of
> free safety and security 
> > tools, free access to millions of high-quality
> videos from across the web, 
> > free AOL Mail and more.
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> >
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 




------------------------------

Message: 19
Date: Thu, 22 Feb 2007 15:56:11 -0800
From: "M C" <mc549 <@t> hotmail.com>
Subject: [Histonet] I have an E300
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY133-F172762998CAA32895D9852F98F0 <@t> phx.gbl>
Content-Type: text/plain; format=flowed

Is anyone in the market for an ASP-300 Tissue Processor.

Contact: mc549 <@t> hotmail.com for more details.

-Marco Corona

_________________________________________________________________
Want a degree but can't afford to quit? Top school degrees online - in as 
fast as 1 year 
http://forms.nextag.com/goto.jsp?url=/serv/main/buyer/education.jsp?doSearch=n&tm=y&search=education_text_links_88_h288c&s=4079&p=5116




------------------------------

Message: 20
Date: Thu, 22 Feb 2007 18:35:41 -0600
From: "Bonnie Whitaker" <bwhitaker <@t> brownpathology.com>
Subject: RE: [Histonet] LIS programs
To: "'Marilyn McDonald'" <kildee6093 <@t> mac.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000e01c756e2$8c8df660$3601a8c0 <@t> brownpathology.net>
Content-Type: text/plain;	charset="us-ascii"

We LOVE WinSurge!!  (If you are asking for Anatomic Path only).  Feel free
to contact me with any particular questions.

Bonnie Whitaker
Lab Manager
Brown & Associates Medical Laboratories
8076 El Rio
Houston, Texas  77054
713-741-6677



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marilyn
McDonald
Sent: Thursday, February 22, 2007 5:47 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] LIS programs


What is your favorite Pathology program? What do you like about it?   
I am familiar with Tamtron (Powerpath).  A friend has asked me so I  
decided to post it on the Histonet.  No vendors please.
Thanks


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 21
Date: Thu, 22 Feb 2007 18:39:54 -0600
From: "Joe Nocito" <jnocito <@t> satx.rr.com>
Subject: Re: [Histonet] PAS stain for nail fungus
To: "Akemi Allison-Tacha" <akemiat3377 <@t> yahoo.com>,
	<rsrichmond <@t> aol.com>,	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001001c756e3$23859370$649eae18 <@t> yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

we tried Nair, but we are getting results with the 20% NH3OH

JTT
----- Original Message ----- 
From: "Akemi Allison-Tacha" <akemiat3377 <@t> yahoo.com>
To: "Joe Nocito" <jnocito <@t> satx.rr.com>; <rsrichmond <@t> aol.com>; 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, February 22, 2007 5:51 PM
Subject: Re: [Histonet] PAS stain for nail fungus


> Hi Joe,
>
> I guess the histology field still uses the old
> methods.  I was taught that back in 1974 at OHSU.
> FYI-We also used soak in NAIR HAIR REMOVAL to soften
> nails.
> Akemi Allison-Tacha
>
>
> --- Joe Nocito <jnocito <@t> satx.rr.com> wrote:
>
>> Hey Doc,
>> you have the South Texas Toenail lab guy right here.
>> That's why I'm known as
>> "Joe the Toe".
>>     We soak our nails in 20% ammonium hydroxide for
>> at least an hour before
>> we put the blocks on the machine to process. We've
>> been getting nailed for a
>> couple of years and perform a PAS for Fungus on each
>> case that we do.
>> Reimbursement must be good, we keep getting nailed.
>>
>> Joe the Toe or just JTT
>>
>> ----- Original Message ----- 
>> From: <rsrichmond <@t> aol.com>
>> To: <histonet <@t> lists.utsouthwestern.edu>
>> Sent: Thursday, February 22, 2007 12:09 PM
>> Subject: [Histonet] PAS stain for nail fungus
>>
>>
>> > Dr. Allen Smith's recent post reminds me of a
>> question I've been meaning
>> > to post to Histonet.
>> >
>> > Podiatrists are now performing biopsies of
>> toenails with nail fungus, in
>> > order to get a fungal stain (PAS is being
>> requested, I think) done.
>> > Apparently the insurance companies are now
>> requiring a biopsy or culture
>> > diagnosis of fungal disease before they'll pay for
>> the very expensive
>> > systemic drugs for nail fungus like terbinafine
>> (Lamisil).
>> >
>> > Is anyone on Histonet getting these requests? What
>> protocols do you use to
>> > cut and stain these difficult specimens?
>> >
>> > Bob Richmond
>> > Samurai Pathologist
>> > Knoxville TN
>> >
>>
> ________________________________________________________________________
>> > Check out the new AOL.  Most comprehensive set of
>> free safety and security
>> > tools, free access to millions of high-quality
>> videos from across the web,
>> > free AOL Mail and more.
>> > _______________________________________________
>> > Histonet mailing list
>> > Histonet <@t> lists.utsouthwestern.edu
>> >
>>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 




------------------------------

Message: 22
Date: Fri, 23 Feb 2007 05:59:51 -0500
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: RE: [Histonet] FDA approved recyclers
To: "'Rene J Buesa'" <rjbuesa <@t> yahoo.com>,	"'Mrosla, Tina M'"
	<tmmrosla <@t> healtheast.org>,	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000701c75739$bea957f0$86f62d4b <@t> HPPav2>
Content-Type: text/plain;	charset="iso-8859-1"

Going to put in my 2 cents, which may not be too accurate.

The FDA (Food and Drug Administration) covers topics that relate to Food and
Drugs and medical devices that go INTO a HUMAN BEING, and anything else that
touches these foods and drugs and medical devices that could effect a
person's health.

So as far as "recycling" would go, the FDA would be concerned about food or
drugs that were being gathered after NOT being used and "recycled" for reuse
(think of drugs after expiration date, or that had already been given to a
patient and returned back to pharmacy). The FDA is also concerned about the
plastic that goes around the food and drugs, as I remember they were
concerned about new "recycled" plastic that the food and drugs are packaged
in, and whether recycled plastic emit anything that would compromise the
food or drugs and make it a health hazard.

I know the FDA was concerned about medical devices (artificial hips, pace
makers, etc.) being removed from people, cleaned up, and "recycled" for use
in another person.

As for a xylene/alcohol/formalin recylcer - I personally think this is out
of the jurisdication of the FDA. The equipment itself does not go inside a
person, nor is the intended use of these chemicals that are being recycled
intended to human consumption. Neither the recycler nor the chemicals are
intended for human USE.

On the other hand, 
- OSHA has lots laws about chemical exposure to people that would apply
- EPA would be concerned if the recycled chemicals got into the atmosphere
or the water, and care about the proper disposal of waste
- CAP has checklist questions for safety and use of equipment, electricity,
chemicals, disposal, etc. that would apply.
- I've never read the GLP, put I'm certain they have similar rules about
chemicals and equipment.
- Local fire marshals and the hospital's insurance company may have their
own concern, especially as to where the recycler is positioned - but this is
for scenarios of catching on fire and explosions.

If someone knows of the FDA's concern in recycling chemicals in the
laboratory, please inform HistoNet. Thanks.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, February 22, 2007 12:50 PM
To: Mrosla, Tina M; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] FDA approved recyclers

Tina:
  I also used a B/R recycler for more than 10 years, we were inspected
frequently in our hospital and we were never told of a FDA requirement.
  If that information cost you any money, ask for your money back!
  René J.

"Mrosla, Tina M" <tmmrosla <@t> healtheast.org> wrote:
  
We were just informed that some hospitals don't recycle xylene, alcohol, or
formalin because solvent recyclers are not approved by the FDA for use in
hospitals. Is this true? 
We have a B/R Procycler 9700 that we have been using for 5 years. Does
anyone know anything about this recycler and FDA approval?

Tina Mrosla, HTL (ASCP)
Histotechnologist
St. Joseph's Hospital
Healtheast Care System
651-232-3575
fax 232-4543
tmmrosla <@t> healtheast.org

Passion for Caring and Service



The information included in this e-mail message, including any attachments,
is intended only for the person or organization to which it is addressed.
This e-mail message may contain information that is privileged or
confidential. If you receive this e-mail message and are not the intended
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included in this e-mail and any attachments. If you received this e-mail
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---------------------------------
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------------------------------

Message: 23
Date: Fri, 23 Feb 2007 06:24:22 -0500
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] LIS programs
To: "Marilyn McDonald" <kildee6093 <@t> mac.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<1CD6831EB9B26D45B0A3EAA79F7EBD32037D34F6 <@t> sjhaexc02.sjha.org>
Content-Type: text/plain;	charset="us-ascii"

Triple G is NOT a good system. I hear that CoPath is the Mercedes of AP
programs. 

Good luck!

Joyce


Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
404-851-7376 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marilyn
McDonald
Sent: Thursday, February 22, 2007 6:47 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] LIS programs

What is your favorite Pathology program? What do you like about it?   
I am familiar with Tamtron (Powerpath).  A friend has asked me so I
decided to post it on the Histonet.  No vendors please.
Thanks


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc.



------------------------------

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