AW: [Histonet] Stain for cerebral blood vessels
gu.lang <@t> gmx.at
Thu Feb 22 12:54:53 CST 2007
I think vanGieson is the shortest collagen-stain. Staining with FGF or
Anilinblue alone would perhaps lead to an overall stain, more in the
collagen fibers less in the rest of the tissue.
These are links to the excellent pathopic-picture-archive. Perhaps you find
the right stain for your needs.
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Laura
Gesendet: Donnerstag, 22. Februar 2007 19:13
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Stain for cerebral blood vessels
I'm a molecular biologist working at the University of Cambridge, UK. I
was wondering if anyone could recommend a histochemical stain that would
work for cerebral blood vessels, preferably in frozen sections? I've
been trying alkaline phosphatase staining but with mixed results.
Would a collagen stain like Fast Green FGF work in isolation (rather
than as part of a trichrome stain)?
It doesn't need to be completely specific for vessels, as long as they
are clearly visible. The other point is that is needs to be as quick and
simple as possible, as I need to extract protein from the tissue
afterwards. Immunostaining is no good for this application. All
suggestions welcome as I will probably need to test several things to
find one that works.
Best wishes, Laura Harris
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