[Histonet] Glycolmethacrylate and antibodies

[Adam Kirk]

Dear histonetters,

I am using aquaporin 2 antibodies that are working in paraffin but not in
GMA. I understand it's a resin and its permeation into the tissue is very
good. It seems in this case too good and is consequently masking the
antigenic site, or reacting with the antibody to prevent its identification
of the aquaporin protein. HAs anyone got any ideas to de-mask when tissue is
embedded in GMA?

Thanks

Adam

-- 
Dr Adam Kirk MRCP
Renal/GIM Registrar
07966015047