[Histonet] Glycolmethacrylate and antibodies

Adam Kirk apothad <@t> gmail.com
Thu Feb 22 08:37:00 CST 2007

Dear histonetters,

I am using aquaporin 2 antibodies that are working in paraffin but not in
GMA. I understand it's a resin and its permeation into the tissue is very
good. It seems in this case too good and is consequently masking the
antigenic site, or reacting with the antibody to prevent its identification
of the aquaporin protein. HAs anyone got any ideas to de-mask when tissue is
embedded in GMA?



Dr Adam Kirk MRCP
Renal/GIM Registrar

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