[Histonet] astrocytes in suspension

[Black, Carolyn]

Hi All,

 

I am fairly new to using any kind of histology methods at all
(engineering background) so I was hoping someone could help me out.  I
am interested in staining astrocytes that are in suspension with a
fluorescent tag so I can then compare amounts of fluorescence between
samples with a fluorometer.  So, I know I would use GFAP (correct me if
I'm wrong) and a fluorescent secondary would fluoresce what was tagged
with GFAP.  Would I have to fix these cells?  If not, would I use some
sort of live cell stain, like GFP?  Please help me out, I'm at a loss!

 

Thanks!

 

Carolyn Black

cblack <@t> med.wayne.edu