[Histonet] IHC-CD117 (c-kit)

[Rene J Buesa]

I never used prediluted antibodies. For CD117 I used the concentrated Ab from Dako, diluted 1:50 with HIER at pH6
  Try to buy the concentrated Ab.
  René J.

"W. Ozanne" <wayneozanne <@t> sympatico.ca> wrote:
  We are using the Dako c-kit with the predilute antibody. We are staining on
the Dako Autostainer using the Dako Target Retrieval, Envision + detection
kit and DAB+ chromogen. We are experiencing inconsistent staining with the
positive control slide provided with the kit as well as a known positive
tissue control that we use.

Today the positive control slide was negative, the known positive patient
case control slide containing a previously identified GIST was very weakly
positive (however the mast cells were nicely positive).

Any suggestions or helpful hints from my colleagues would be so greatly
appreciated.

Thanks,


Wayne Ozanne
Chief Technologist, Pathology Department
St. Joseph's Health Centre
Toronto, ON

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