[Histonet] primary incubation times
making <@t> ufl.edu
Thu Feb 15 13:17:16 CST 2007
I remember reading in one of the early immunohistochemistry texts that
the reaction between a primary antibody and its epitope was believed to
require 96 hrs. at 4 deg. C to reach equilibration. Whether or not
that's true for your antibodies, it is the kind of precedent that
underlies the procedures typically used today.
The other issue is penetration--if you need tissue to be evenly labeled
throughout the depth of the specimen then section thickness and
embedding material may require long incubations (at least). If surface
labeling of an abundant epitope is enough to tell you what you want to
know, a good primary may require less than half an hour!
Date: Wed, 14 Feb 2007 15:24:53 -0800
From: "Patrick Laurie" <plaurie <@t> benaroyaresearch.org>
Subject: [Histonet] Reason for lengthy primary antibody incubation?
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