[Histonet] GFP antibody

[Cynthia A Delong]

Hello all,
I am looking for a good working protocol for GFP antibody with possibly a 
photo.  I am working a little blind because of no control. 
I am under the understanding that when you fix the GFP mouse that there is 
a loss of staining.  My question is can you pull it back out with a GFP 
antibody since the sites should still be there. 
I have non fixed frozen brain sections cut at 10 microns.
Any advice would be appreciated.
Thank you,
Cindy