[Histonet] rabbit noggin antibody

koellingr <@t> comcast.net koellingr <@t> comcast.net
Tue Feb 13 08:33:51 CST 2007

I realize you asked about anti-rabbit noggin but 2 professional lives ago, I used a great anti-mouse noggin for both Westerns and IHC.  From R&D Systems it was a goat anti-mouse noggin.  Worked well in my Westerns down to a few ng detection.  Since it worked well under both denatured and non-denaturing conditions, thought of it for IHC.  Indeed it worked in frozen and also paraffin with retrieval.  Just went after it with an anti-goat secondary pre-absorbed to mouse.  Mammalian noggin is nearly homologous to even something like Xenopus noggin and indeed since many embryonic spacial-fate specification molecules are conserved across species, the rabbit noggin target (I never attempted)should be quite similar to mouse noggin (I did successfully in Western and IHC).  If no one answers specifically regarding rabbit noggin antibodies, you might give the R&D Systems goat anti-mouse noggin a try.  Seems like a logical and reasonable attempt to me.

Raymond Koelling
Currently Employment Challenged
Seattle, WA 

-------------- Original message -------------- 
From: Caroline Bass <cbass <@t> bidmc.harvard.edu> 

> Hello, 
> Can anyone recommend a good antibody for noggin? It will be used for 
> western blot and staining in rabbit tissues. 
> Thanks, 
> Caroline 
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