[Histonet] 33D1 DC marker- further info
sonya.martin <@t> soton.ac.uk
Wed Feb 7 06:42:18 CST 2007
Some further info;
The sections were mouse spleen - we did some flow analysis with 33D1 of
splenocytes but didnt see anything - hard to know if this is because
there are just so few 33D1+ cells or if theres a problem with the
antibody. There are a few papers out there with quite a lot of staining
with 33D1 in mouse spleen - most recently in Science (Dudziak et al Vol
315 Jan 2007). For frozen sections I have tried 33D1 + mouse anti-rat
IgG2b:Biotin with Streptavidin Alexa488 and also 33D1 + rb
anti-rat:Biotin with tyramide amplification and Streptavidin Alexa488.
The coverslip DC's were BMDC's - BM cells were harveted from mice and
grown with GMCSF for 5 days before transfering cells to PLL coated
coverslips. Cells were allowed to attach for 2hrs and were then fixed
with 4% PFA, 10min.
Basically we want to look at DC's in the spleen in conjuction with a
number of other cell markers. We are using CD11c and Dec205 antibodies
but wanted an additional DC marker.
Thanks for all your suggestions/comments
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