[Histonet] peptide-ab blocking

[Carlos G. Perez-Garcia]

hi everybody

i need to do a peptide-antibody blocking experiment to check the  
specificity of an antibody i made. i wonder if somebody has  
experience on that. i suppose that just adding 5-10 times more  
peptide than antibody in the solution will be enough right? does  
anybody have any protocol or suggestion?

thanks in advance

Carlos


Carlos G. Perez-Garcia, Ph.D.

Carlos G. Perez-Garcia, Ph.D.
Molecular Neurobiology Lab (MNL-O)
The Salk Institute
10010 North Torrey Pines Road
92037 La Jolla, CA
USA

cpgarcia <@t> salk.edu
Fax: 858 558 6207