[Histonet] peptide-ab blocking

Carlos G. Perez-Garcia cpgarcia <@t> salk.edu
Mon Feb 5 12:21:46 CST 2007

hi everybody

i need to do a peptide-antibody blocking experiment to check the  
specificity of an antibody i made. i wonder if somebody has  
experience on that. i suppose that just adding 5-10 times more  
peptide than antibody in the solution will be enough right? does  
anybody have any protocol or suggestion?

thanks in advance


Carlos G. Perez-Garcia, Ph.D.

Carlos G. Perez-Garcia, Ph.D.
Molecular Neurobiology Lab (MNL-O)
The Salk Institute
10010 North Torrey Pines Road
92037 La Jolla, CA

cpgarcia <@t> salk.edu
Fax: 858 558 6207

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