[Histonet] ER control tissue

[Bryan Hewlett]

René,

Dako DOES specify fixation time, i.e. 16-24 hours in NBF prior to processing!
I suspect that not many clinical labs follow this !
If they did, then the target would be much more reproducibly demonstrated!

Bryan

  ----- Original Message ----- 
  From: Rene J Buesa 
  To: Joe Nocito ; Bryan Hewlett ; Richard Cartun ; bamoe <@t> gundluth.org ; histonet <@t> lists.utsouthwestern.edu 
  Sent: Sunday, February 04, 2007 1:38 PM
  Subject: Re: [Histonet] ER control tissue


  I agree that there is no better control tissue for an inhouse IHC test than a positive control processed just like the tissue to be tested BUT if this approach is to be always used, where this "connects" with tests like the Her2Neu provided by Dako with a FAD approved protocol that uses as controls their cultured cells in pellets processed in a different way?
  Either the FDA will have to extend approval to our processing protocols and to used our own positive cases, or Dako has to expla in their processing protocol to be followed by all labs (fat change of either!).
  Just a thoght!
  René J.

  Joe Nocito <jnocito <@t> satx.rr.com> wrote:
    wouldn't using someone else's tissue kind of invalidate those trying to 
    establish ASRs? Just wondering.

    Joe
    ----- Original Message ----- 
    From: "Bryan Hewlett" 
    To: "Richard Cartun" ; ; 

    Sent: Sunday, February 04, 2007 11:33 AM
    Subject: Re: [Histonet] ER control tissue


    > Hi Rich,
    >
    > I completely agree with your final sentence.
    > However, complete in-house standardization of IHC testing (HER2, EGFr, ER, 
    > PR, CD20, CD117, etc.),
    > absolutely has to include standardization of the in-house fixation and < BR>> processing!
    > Only once this has been achieved, and the controls in use are prepared in 
    > a truly similar manner to the test material,
    > will we be able to have a standardized IHC test!
    > Anything less means that we will continue to be shooting at a wildly 
    > moving target.
    >
    > Best regards,
    > Bryan
    >
    > ----- Original Message ----- 
    > From: "Richard Cartun" 
    > To: ; 
    > Sent: Sunday, February 04, 2007 11:58 AM
    > Subject: Re: [Histonet] ER control tissue
    >
    >
    > As we move forward with standardization in IHC testing (HER2, EGFr, ER, 
    > PR, CD20, CD117, etc.), we really need to start thinking about using 
    > control tissue (including TMAs) fixed and processed in your own 
    > laboratory. Although useful for proficiency testing, results obtained on 
    > someone else's t issue will not completely validate your testing.
    >
    > Richard
    >
    > Richard W. Cartun, Ph.D.
    > Director, Immunopathology & Histology
    > Assistant Director, Anatomic Pathology
    > Hartford Hospital
    > 80 Seymour Street
    > Hartford, CT 06102
    > (860) 545-1596
    > (860) 545-0174 Fax
    >
    >>>> 02/01/07 4:41 PM >>>
    >
    >
    >
    >
    > Hello all -
    >
    > Does anyone know of a source to order ER controls for IHC -- other 
    > than
    > Pantomics in California? Ideally I'm looking for an array that would
    > contain a strong (3+), moderate (2+), and low/negative (1+) 
    > representation.
    >
    > Thanks for any help!
    >
    > Barb Moe
    > Gundersen Lutheran Medical Center
    > La Crosse WI
    >
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