[Histonet] peptide-blocking

Carlos G. Perez-Garcia cpgarcia <@t> salk.edu
Sat Feb 3 19:35:05 CST 2007


hi everybody

i need to do a peptide-antibody blocking experiment to check the  
specificity of an antibody i made. i wonder if somebody has  
experience on that. i suppose that just adding 5-10 times more  
peptide than antibody in the solution will be enough right? does  
anybody have any protocol or suggestion?

thanks in advance

Carlos


Carlos G. Perez-Garcia, Ph.D.







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