[Histonet] peptide-blocking

[Carlos G. Perez-Garcia]

hi everybody

i need to do a peptide-antibody blocking experiment to check the  
specificity of an antibody i made. i wonder if somebody has  
experience on that. i suppose that just adding 5-10 times more  
peptide than antibody in the solution will be enough right? does  
anybody have any protocol or suggestion?

thanks in advance

Carlos


Carlos G. Perez-Garcia, Ph.D.