[Histonet] A question..

[Dawson, Glen]

Rosa,

One of the main things is that the detergent in buffer breaks down surface tension allowing subsequent reagent to spread more evenly over the whole slide.  It isn't necessary to "soak" them for an extended period of time but I've noticed that, with water only, reagent tends to bead up which can lead to some of the tissue on the slide receiving inadequate treatment.

Glen Dawson
IHC Manager
Milwaukee, WI

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Rosa
Bryant
Sent: Friday, February 02, 2007 10:34 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] A question..


What is the science behind soaking the slides in buffer before IHC procedures?  I can't find much of a reference about this, what are the advantages to this step instead of putting the slides back into water?  Thanks  Rosa Fields 
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