[Histonet] Sticky Paraffin

Rene J Buesa rjbuesa <@t> yahoo.com
Fri Feb 2 09:34:53 CST 2007


Usually paraffin of low melting point is appreciated as sticky. It could be that you have received a batch of lower than usual melting point, or that your paraffin is being contaminated with the antemedium. Also higher than usual temperatures in the sectioning area causes the paraffin to appear sticky.
  René J.

"Inman, Anna" <Anna.Inman <@t> stmarygj.org> wrote:
  Happy Friday Histonetters! 
Recently, our techs have been encountering what is termed here as
"sticky paraffin." It is sticking more than usual to the embedder and
more importantly to the cutters and creating difficulties at the
microtome. We are also encountering much more scratchy tissue that will
not ribbon appropriately and I am not sure if the problems are related
(maybe a processing issue or entirely separate issues??). It is not
everyday but seems to be increasingly worse. 
We are using Richard Allen Type 9 media. The question has been posed
...should we be using a paraffin with less polymers? 
Could the sticky paraffin be a result of the weather (air too dry?) 

Any thoughts? 

Thank you all for your help! 

Anna Inman, HT(ASCP)
Clinical Resource Specialist
SMH Pathology
Anna.Inman <@t> stmarygj.org



CONFIDENTIALITY NOTICE:This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




 
---------------------------------
Any questions?  Get answers on any topic at Yahoo! Answers. Try it now.


More information about the Histonet mailing list