[Histonet] EBER

Dana Settembre settembr <@t> umdnj.edu
Thu Feb 1 14:39:26 CST 2007

Hello Patti,
I use Dako's PNA ISH Detection kit Cat# K5201
along with their EBV EBER PNA probe Cat# Y5200
Their protocol is very easy to use. It is a one day run.

Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA

>>> Patti Loykasek <ploykasek <@t> phenopath.com> 02/01/07 2:20 PM >>>
I have an inquiry for anyone doing EBV in-situ hybridization. What type
probe are you using? How is your probe labeled? Are you using a kit &
if so,
what kit? We are looking to optimize our sensitivity. Currently we are
a digoxigen labeled EBV probe that we have made for us, detection is
via an
anti-dig link and then Envision+-HRP, followed by DAB.
Thanks for the info.

Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA

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