[Histonet] mitachrondrial disease

[Robert Chiovetti]

Patsy,

Is EM a possibility?  The mitochondria in patients w/ some of the inherited mito diseases can be really strange-looking.  If that's not possible, maybe some special staining can help.

Gomori trichrome or even H&E can show "ragged red fibers" (probably due to the overabundance of mitochondria).  Sudan can stain excess lipid (if it's there).  Cytochrome oxidase (COX) can be reduced or absent in some mito diseases, and It looks like there can also be differences in succinic dehydrogenase (SDH) staining.

Take a look at:



<http://www.neuro.wustl.edu/neuromuscular/pathol/mitochondrial.htm>


...just my 2 cents' worth...

Bob


Robert (Bob) Chiovetti, Ph.D.

Southwest Precision Instruments
www.swpinet.com
Arizona's Microscopy Resource
132 North Elster Drive
Tucson, AZ 85710-3212
Tel./Fax 520-546-4986
Member, Arizona Small Business Association
(www.asba.com)

----- Original Message ----
From: "pruegg <@t> ihctech.net" <pruegg <@t> ihctech.net>
To: Histonet <histonet <@t> pathology.swmed.edu>
Sent: Friday, December 21, 2007 9:34:57 PM
Subject: [Histonet] mitachrondrial disease


Can someone recommend a histology approach to demonstrate diseased
mitochondria, the investigators I am working with are cardiologist
 dealing
with genetic defects.

Thank you and happy holidays,

Patsy 

 

 

 

Patsy Ruegg, HT(ASCP)QIHC

IHCtech, LLC

Fitzsimmons BioScience Park

12635 Montview Blvd. Suite 215

Aurora, CO 80010

P-720-859-4060

F-720-859-4110

email pruegg <@t> ihctech.net

website www.ihctech.net

IHC Resource Group www.ihcrg.org 

 

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