[Histonet] Rat T Cell Marker

[Tarango, Mark]

SATB1 is a good nuclear marker for T-cells.  I've only used it on human
tissue though.  I bet you can find an anti- rat SATB1.


Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV  89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663
  

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kristen
Broomall
Sent: Wednesday, December 19, 2007 6:03 AM
To: Histonet
Subject: [Histonet] Rat T Cell Marker

Good Morning all, I was wondering if anyone had suggestions on an
antibody
to detect T cells (only, no B cells) in formalin fixed, paraffin
embedded
rat thymus and spleen. I have a Pan T Cell marker that is working well
in
the spleen, but so-so in the thymus, so I think that it is only staining
the
mature T cells, and not immature cells (which of course, is what we also
need to see). I've been reading up alot on T cells and trying to figure
this
out (CD3, CD4, CD8, TCR, lions, tigers and bears - Oh My!), but I fear
our
deadline for getting staining done is shorter than the time I have to
educate myself properly!

Any suggestions would be greatly appreciated!


Happy Holidays,
Kristen Broomall, HT (ASCP)
histotechkb <@t> gmail.com
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"EMF <nvcancer.org>" made the following annotations.
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