[Histonet] Rat T Cell Marker

Tarango, Mark mtarango <@t> nvcancer.org
Wed Dec 19 16:45:10 CST 2007

SATB1 is a good nuclear marker for T-cells.  I've only used it on human
tissue though.  I bet you can find an anti- rat SATB1.

Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV  89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kristen
Sent: Wednesday, December 19, 2007 6:03 AM
To: Histonet
Subject: [Histonet] Rat T Cell Marker

Good Morning all, I was wondering if anyone had suggestions on an
to detect T cells (only, no B cells) in formalin fixed, paraffin
rat thymus and spleen. I have a Pan T Cell marker that is working well
the spleen, but so-so in the thymus, so I think that it is only staining
mature T cells, and not immature cells (which of course, is what we also
need to see). I've been reading up alot on T cells and trying to figure
out (CD3, CD4, CD8, TCR, lions, tigers and bears - Oh My!), but I fear
deadline for getting staining done is shorter than the time I have to
educate myself properly!

Any suggestions would be greatly appreciated!

Happy Holidays,
Kristen Broomall, HT (ASCP)
histotechkb <@t> gmail.com
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

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