[Histonet] decalcifiers and Safranin O staining

Michele Wich mwich <@t> 7thwavelabs.com
Wed Dec 19 12:24:19 CST 2007

Can anyone explain the chemistry behind decalcifying agents and the
Safranin O procedure?...Specifically, why does using Immunocal (formic
acid) as a decalcifier yield beautiful Safranin O staining while RDO
(HCL) almost totally eliminates staining? Does HCL do something to the
proteoglycans that formic acid does not?




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