[Histonet] RE: ED1 staining in rat
Wynn, Carmen
Carmen.Wynn <@t> us.astellas.com
Tue Dec 18 17:28:30 CST 2007
Hello Michele,
I have also had good results with the AbD Serotec's mouse anti-rat CD68
(ED1) in rejected grafts of FFPE rat heart and kidneys at 1:100
dilution. I used Trypsin Grade II (sigma cat#7168) for 30 minutes at
37C. I didn't have to use any HIER but as Noelle stated before...it is
a pretty hardy antibody.
Carmen Wynn, M.S., Senior Scientist
Astellas Research Institute of America, LLC. (ARIA)
Illinois Science and Technology Park
8045 Lamon Ave
Skokie, IL 60077
Direct: 847-933-7419
Main: 847-933-7400
Fax: 847-933-7401
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, December 18, 2007 12:18 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 49, Issue 19
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. Bouin's fixation for mouse nasal passages (Helen E Johnson)
2. ED1 staining in rat (Michele Wich)
3. Desmin (Perry, Samuel)
4. RE: ED1 staining in rat (Linke_Noelle)
5. AW: [Histonet] Desmin (Gudrun Lang)
6. Cold Plate (Piche-Grocki, Jessica)
7. Re: Schiff's reagent (Solomon Adebayo)
----------------------------------------------------------------------
Message: 1
Date: Mon, 17 Dec 2007 13:32:54 -0500
From: Helen E Johnson <hej01 <@t> health.state.ny.us>
Subject: [Histonet] Bouin's fixation for mouse nasal passages
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF8D8F07F4.497D4380-ON852573B4.006544A1-852573B4.0065E4AD <@t> notes.health.
state.ny.us>
Content-Type: text/plain; charset=US-ASCII
Hi Histonetters,
How long should mouse nasal passages be fixed in Bouin solution
before cutting in & processing?
Helen Johnson
(hej01 <@t> health.state.ny.us)
IMPORTANT NOTICE: This e-mail and any attachments may contain
confidential or sensitive information which is, or may be, legally
privileged or otherwise protected by law from further disclosure. It is
intended only for the addressee. If you received this in error or from
someone who was not authorized to send it to you, please do not
distribute, copy or use it or any attachments. Please notify the sender
immediately by reply e-mail and delete this from your system. Thank you
for your cooperation.
------------------------------
Message: 2
Date: Mon, 17 Dec 2007 12:48:38 -0600
From: "Michele Wich" <mwich <@t> 7thwavelabs.com>
Subject: [Histonet] ED1 staining in rat
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2264717ADC396742A0FF0AAB674F9A0D486B65 <@t> 7THWAVE-SERVER.7thwave.local>
Content-Type: text/plain; charset="us-ascii"
Does anyone have an IHC protocol for ED1 staining in formalin-fixed
paraffin sections of rat tissue (specifically rat brain)? I've noticed
several mouse anti-rat antibodies available, but I'm not really sure
where to start. Any suggestions would be much appreciated.
~Michele
This communication is intended solely for the use of the addressee and
may contain information that is legally privileged, confidential or
exempt from disclosure. If you are not the intended recipient, please
note that any dissemination, distribution, or copying of this
communication is strictly prohibited. Anyone who receives this message
in error should notify the sender immediately and delete it from his or
her computer
------------------------------
Message: 3
Date: Mon, 17 Dec 2007 13:24:14 -0500
From: "Perry, Samuel" <Samuel_Perry <@t> DFCI.HARVARD.EDU>
Subject: [Histonet] Desmin
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D3C984272CCAA741879C0B9339D1A7670B9006 <@t> PHSXMB24.partners.org>
Content-Type: text/plain; charset="us-ascii"
Hi All,
I am trying to perform IHC staining using the Dako envision+ kit on
mouse and
human tissue. I am looking for an anti-desmin antibody which has been
well
published and characterized in the past. There seems to be several
rabbit
polyclonal antibodies out there by abcam, anyone have luck with these?
I look
forward to hearing peoples responses.
Thanks -Sam Perry
Dana Farber Cancer Institute
Research Technician
The information transmitted in this electronic communication is intended
only
for the person or entity to whom it is addressed and may contain
confidential
and/or privileged material. Any review, retransmission, dissemination or
other
use of or taking of any action in reliance upon this information by
persons or
entities other than the intended recipient is prohibited. If you
received this
information in error, please contact the Compliance HelpLine at
800-856-1983 and
properly dispose of this information.
------------------------------
Message: 4
Date: Mon, 17 Dec 2007 10:58:01 -0800
From: "Linke_Noelle" <Linke_Noelle <@t> Allergan.com>
Subject: RE: [Histonet] ED1 staining in rat
To: "Michele Wich" <mwich <@t> 7thwavelabs.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5C3DA4BE34AA0641BAA10A7C1478B60527CFED <@t> IRMAIL132.irvine.allergan.com>
Content-Type: text/plain; charset="iso-8859-1"
That's a pretty easy one to get working....
ED1 from Serotec (cat# MCA341R), 1:400 dilution, citrate pretreatment
(this Ab is pretty strong, may work without it) 1 hour incubation.
Noelle
Nolle Linke, MS, HTL(ASCP)QIHC
Allergan, Inc
2525 Dupont Drive RD-2A
Irvine, CA 92612
714-246-5568
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michele
Wich
Sent: Monday, December 17, 2007 10:49 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] ED1 staining in rat
Does anyone have an IHC protocol for ED1 staining in formalin-fixed
paraffin sections of rat tissue (specifically rat brain)? I've noticed
several mouse anti-rat antibodies available, but I'm not really sure
where to start. Any suggestions would be much appreciated.
~Michele
This communication is intended solely for the use of the addressee and
may contain information that is legally privileged, confidential or
exempt from disclosure. If you are not the intended recipient, please
note that any dissemination, distribution, or copying of this
communication is strictly prohibited. Anyone who receives this message
in error should notify the sender immediately and delete it from his or
her computer
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Tue, 18 Dec 2007 08:03:57 +0100
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] Desmin
To: "'Perry, Samuel'" <Samuel_Perry <@t> DFCI.HARVARD.EDU>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <001401c84144$29ea9480$eeeea8c0 <@t> dielangs.at>
Content-Type: text/plain; charset="iso-8859-1"
We use monoclonal mouse Desmin D33 from Dako, 1:100 on Ventana Benchmark
(pH
8 retrieval; 24 min inkubation).
Gudrun Lang
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Perry,
Samuel
Gesendet: Montag, 17. Dezember 2007 19:24
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Desmin
Hi All,
I am trying to perform IHC staining using the Dako envision+ kit on
mouse
and
human tissue. I am looking for an anti-desmin antibody which has been
well
published and characterized in the past. There seems to be several
rabbit
polyclonal antibodies out there by abcam, anyone have luck with these?
I
look
forward to hearing peoples responses.
Thanks -Sam Perry
Dana Farber Cancer Institute
Research Technician
The information transmitted in this electronic communication is intended
only
for the person or entity to whom it is addressed and may contain
confidential
and/or privileged material. Any review, retransmission, dissemination or
other
use of or taking of any action in reliance upon this information by
persons
or
entities other than the intended recipient is prohibited. If you
received
this
information in error, please contact the Compliance HelpLine at
800-856-1983
and
properly dispose of this information.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Tue, 18 Dec 2007 12:40:41 -0500
From: "Piche-Grocki, Jessica" <jpiche-grocki <@t> wtbyhosp.org>
Subject: [Histonet] Cold Plate
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0A57D6AEAE4CBA4A984D27257160A72D01069D22 <@t> win03exchange01.wtbyhosp.org>
Content-Type: text/plain; charset="iso-8859-1"
Hi,
Just wanted to say thanks to everyone who shared information on cold
plate QC. Thanks again,
Jessica
CONFIDENTIALITY NOTICE: This email and any attachments contain
confidential information that is legally privileged. This information is
intended only for the use of the individual or entity named above. The
authorized recipient of this information is prohibited from disclosing
this information to any other party unless required to do so by law or
regulation.
If you are not the intended recipient, you are hereby notified that any
disclosure, copying, distribution or action taken in reliance on the
contents of these documents is strictly prohibited. If you have received
this information in error, please notify the sender immediately and
delete these documents. Copyright (c) Waterbury Hospital
------------------------------
Message: 7
Date: Tue, 18 Dec 2007 09:57:07 -0800 (PST)
From: Solomon Adebayo <benardsolomona <@t> yahoo.com>
Subject: Re: [Histonet] Schiff's reagent
To: Andrea Grantham <algranth <@t> u.arizona.edu>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <450981.38163.qm <@t> web36105.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
This is a rather late reply to the querry by Andrea.
The shell life and stability of schiff's reagent
depend on the formular and mode of preparation.
we use a modified schiff's reagent with a shell life
of more than 12 months. It still maintain its potency
and very stable.
Benard Solomon
Pathology Dept.,
Unilorin Teaching Hospital,
Ilorin, Kwara State,
Nigeria
www.histosearch/homepage/benardsolomon
--- Andrea Grantham <algranth <@t> u.arizona.edu> wrote:
> I'm using a Schiff's from Newcomer Supply that
> has a pretty long shelf life. It can be stored at
> RT and can be used several times.
>
> We always put the "used" Schiff's into a
> different bottle as to not contaminate the unused
> solution.
>
> Andi Grantham
>
>
>
>
> At 03:29 PM 12/3/2007, Rene J Buesa wrote:
> >It has to be kept in the refrigerator, and yes, it
> can be used several times.
> > You can test how is working by adding a few
> > drops of the Schiff's reagent to NBF, it it
> > reacts quickly with a magenta color, is working
> OK, if not, discard it.
> > Ren J.
> >
> >Taben Hale <taben.hale <@t> gmail.com> wrote:
> > What is the shelf-life and stability of Schiff's
> reagent? Can it be used
> >multiple times?
> >
> >--
> >Taben M Hale, PhD
> >Postdoctoral Fellow
> >Universite de Montreal
> >Dept Pharmacologie
> >(514)343-6111 ext. 4968
> >_______________________________________________
> >Histonet mailing list
> >Histonet <@t> lists.utsouthwestern.edu
>
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> >---------------------------------
> >Be a better sports nut! Let your teams follow
> >you with Yahoo Mobile. Try it now.
> >_______________________________________________
> >Histonet mailing list
> >Histonet <@t> lists.utsouthwestern.edu
>
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
.....................................................................
> : Andrea Grantham, HT(ASCP) Dept. of Cell
> Biology & Anatomy :
> : Sr. Research Specialist University of
> Arizona :
> : (office: AHSC 4212) P.O. Box 245044
> :
> : (voice: 520-626-4415) Tucson, AZ
> 85724-5044 USA :
> : (FAX: 520-626-2097) (email:
> algranth <@t> u.arizona.edu) :
>
:...................................................................:
>
> http://www.cba.arizona.edu/histology-lab.html
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
________________________________________________________________________
____________
Looking for last minute shopping deals?
Find them fast with Yahoo! Search.
http://tools.search.yahoo.com/newsearch/category.php?category=shopping
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 49, Issue 19
****************************************
More information about the Histonet
mailing list