[Histonet] frozen section temperature chart

[Kim Merriam]

Thanks so much for all of the responses I got.  It seems to me that the best way to get this chart is in your cryostat operators manual.  The Leica website has many manuals on-line and I managed to find one here (page 52):
http://www.histo-solutions.com/pdfs.nsf/(ALLIDs)/8E60A5111F8D6CC3C12573920052A4C6/$FILE/Leica_CM1950_Manual_1v1_EN.pdf

This seems to be an issue that should really be addressed in more textbooks.  I looked through all of the ones that I had and the only textbook that mentioned it was Bancroft, but this chart was very generic with very wide temperature ranges.

Thanks again,
Kim


 
Kim Merriam, MA, HT(ASCP)
Cambridge, MA



----- Original Message ----
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
To: Andrea Grantham <algranth <@t> u.arizona.edu>
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>; histonet-bounces <@t> lists.utsouthwestern.edu
Sent: Monday, December 17, 2007 11:02:23 AM
Subject: Re: [Histonet] frozen section temperature chart

There is also a chart in the Bancroft and Gamble book.  Theory and 
Practice of Histological Techniques, page 102.

Jennifer MacDonald
Director, Histotechnician Training Program
Mt. San Antonio College
1100 N. Grand Ave.
Walnut, CA 91789
(909) 594-5611 ext. 4884
jmacdonald <@t> mtsac.edu



Andrea Grantham <algranth <@t> u.arizona.edu> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
12/17/2007 06:06 AM

To
Histonet <histonet <@t> lists.utsouthwestern.edu>
cc

Subject
Re: [Histonet] frozen section temperature chart






There was a chart like this in the procedure manual here when I came 
and it is from Reichert-Jung. Must have come in the operation manual 
for one of the cryostats they used to have here.

Andi



At 05:47 AM 12/17/2007, Kim Merriam wrote:
>Hi,
>
>Does anyone know where I can access a chart (it is for an SOP) that 
>has a list of the recommended cryostat temperatures for cutting 
>certain types of tissues.
>
>Thanks in advance,
>Kim
>
>Kim Merriam, MA, HT(ASCP)
>Cambridge, MA
>
>
> 
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