[Histonet] Antigen Retrieval

[Anna Elisse Beaudin]

hi Jackie,

  I do 10 minutes in 95C citrate buffer pH6.0, followed by 20 minutes
cooling at room temp.  I'm working with 20 uM mouse embryo sections, and
it seems to do the trick for Ki-67 immunohistochemistry.  hope that
helps,

Anna



 If anyone could recommend a method for antigen retrieval for Ki-67 on
> rat and mouse tissue that has been fixed in formalin for more than 90
> days, I would really appreciate it.
>
> Jackie Crofoot
> Dow Chemical Co.
> 1803 Building
> Pathology Dept.
> Midland, MI  48674
> Tx 989-636-3539
>
>
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