[Histonet] prolonged impregnation in Golgi-Cox (Kolb) staining
Olek Michalski
olek.michalski <@t> nencki.gov.pl
Thu Dec 13 14:31:22 CST 2007
Dear Histonetters,
I have some brains which were left in Golgi-Cox chromation solution
(K2Cr2O7, KCrO3, HgCl2: about 1% of each) for over 6 months. I would
really like to use this material even if staining would not be very clear.
Do you have an idea how to process the tissue in this case? Any help would
be appreciated (and I know, next time I will be more careful about where I
leave my preparates).
By the way, I have already cut one of these brains and I found the
sections to be dark green. I have noticed this colour in my G-C preparates
before but mainly on the surface. I am actually curious whether the tissue
should be more green or more red when I cut it. I mean should I shorten
the normal period of chromation (about 14-15 days) if I see the brain is
getting green?
Yours truly
Olek Michalski
--
Laboratory of Neurobiology
of Development and Evolution
Nencki Institute of Experimental Biology
ul. Pasteura 3, 02-093 Warszawa, Poland
Tel. +48 22 5892268, Fax +48 22 8225342
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