[Histonet] manual IHC queries
mtarango <@t> nvcancer.org
Wed Dec 12 10:53:19 CST 2007
I do sometimes use coverslips when doing IHC by hand. The humidity in
Las Vegas is pretty low, so evaporation is always an issue. The
Ventana's instruments use a coat of oil, over the aqueous antibody
solution, to prevent evaporation. You could try using a lightweight
mineral oil too.
Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV 89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of louise
Sent: Wednesday, December 12, 2007 12:36 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] manual IHC queries
Firstly: As a matter of interest - when using small amounts of primary
antibody does anyone use coverslips to spread the solution over the
and keep it from evaporating during the incubation? (i do use a humidity
chamber also). I have been doing this for ages and wondering if there
alternative to use other than the coverslips that may be cheaper - any
secondly, Although 30min incubations are recommended, is there any value
increasing incubation times of secondary and ABC complexes to improve
sensitivity? In other words is there a maximal binding period after
improvement will be noted?
I hope I have made myself clear here....these are just passing wednesday
thoughts in preparation for the usual friday meltdown!
Bone Research Unit
University of the Witwatersrand
"There are nights when the wolves are silent and only the moon howls".
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
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