[Histonet] Thanks for answering "section on overfixed paraffin tissue" email

[FU,DONGTAO]

Hi,

  Thank you all for response to my question "section on overfixed 
paraffin tissue". I really appreciate.

  Have a nice day,

Ann


On Mon Dec 10 18:36:09 EST 2007, Mickie Johnson 
<mickie25 <@t> netzero.net> wrote:

> HI Ann,
> 
> This sounds to me a little like over-processed tissue, meaning 
> over
> dehydrated and over cleared. Animal tissue especially is 
> sensitive to
> becoming brittle. Under infiltration could be a problem, but 
> check
> dehydration and clearing times. Good Luck
> 
> Mickie
> 
> Mickie Johnson, B.S., HTL(ASCP)
> Mohs Histology Consulting Services, LLC
>   & Mohs Lab Staffing
> 2507 S. Manito Blvd.
> Spokane, WA 99203
> 509-954-7134
> Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: 
> mickie25 <@t> netzero.net
>   -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
> FU,DONGTAO
> Sent: Monday, December 10, 2007 11:18 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] section on overfixed paraffin tissue
> 
> Hi, all
> 
>   One of our histotechnician met a big problem on sectioning 
> overfixed human and mouse pancreas. The tissues were overfixed in 
> 4%NBF for several weeks before our PI gave to us for paraffin 
> embedding and section. The histotechnician could not get intact 
> pieces of the tissues because the sections started to break when 
> they attach to the water(42-45C) in the waterbath. Does anyone 
> have any experiences on sectioning overfixed tissues? Any 
> suggestions?
> 
>   Thank you,
> 
> Ann Dongtao Fu MD, Ph.D
> Lab Manager
> Molecular Pathology Core
> Dept. of Pathology
> Lab phone: 352-273-7752
> Lab FAX: 352-273-7755
> Lab address: D11-50
> PO Box: 100275
> 1600 SW Archer Road
> University of Flodrida
> Gainesville, FL 32610
> 
> 
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