[Histonet] Re: What is the CAP required water quality for
a histology
Downs, Heather M.
HDOWNS <@t> PARTNERS.ORG
Fri Dec 7 13:14:01 CST 2007
I think what they want you to do is have it cultured to make sure that there is
no bacteria growing in it that could influence a stain. JACHO also has a
requirement that our DI be sent to the micro lab once a month for culture, but
we just supersede it by purchasing DI from a manufacturer, and then the quality
control is on them not us.
Heather Downs
Nerve Injury Unit
Massachusetts General Hospital
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, December 07, 2007 1:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 49, Issue 9
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Today's Topics:
1. Interrupted thionin staining (Nicola J. Broadbent Ph.D)
2. immunohitochem for IFN gamma (Mirela Hasu)
3. Re: reticulin/snooks (Philip Oshel)
4. Patchy staining (Martin, Gary)
5. Re:Interrupted thionin staining (Nicola J. Broadbent Ph.D)
6. RE: Re: Slide quality (Fail, Mildred M.)
7. What is the CAP required water quality for a histology lab?
We have been told we need to test our D (Janice Mitchell)
8. RE: Re: Slide quality (Tony Henwood)
9. Nissl staining question: ignorant question from an ignorant
person (Sharon)
10. Golgi slide bubbles (Bob Nienhuis)
11. Re: What is the CAP required water quality for a histology
lab? We have been told we need to test our D (Joe Nocito)
12. bromephenol blue (Dalia El Rouby)
13. CK8 immunofluorescence problem (Sofia Havaki)
14. c-fos (Valeria Berno)
15. RE: Nissl staining question: ignorant question from an
ignorant person (Smith, Allen)
----------------------------------------------------------------------
Message: 1
Date: Thu, 6 Dec 2007 10:03:15 -0800
From: "Nicola J. Broadbent Ph.D" <nbroadbent <@t> ucsd.edu>
Subject: [Histonet] Interrupted thionin staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002d01c83832$46d7b0c0$8a4cef84 <@t> Nicola>
Content-Type: text/plain; charset="us-ascii"
Hi,
I would like to know whether it is ok to place rat brain tissue (50um slices
on glass gelatin coated slides) directly from xylene to 100% alcholol to
start the thionin staining process. Normally, we defat in a mix of
chloroform and alcohol (approx 1 hr; which we did do with these brain
sections) but due to being evacuated from our building we had to stop the
staining process and in to store the tissue (as we were unsure how long we
would be away from the lab) we placed the tissue in the zylene. The
sections ended up being in zylene for approx 24 hrs. Would it be ok just to
start the staining process from this point i.e. go straight to the 100%, 95%
70% alcohol steps from the zylene?
Thanks so much for your help,
Nicola
Nicola J. Broadbent Ph.D,
Asst. Project Scientist
Department of Psychiatry 0603,
UCSD School of Medicine,
9500 Gilman Dr,
La Jolla, CA, 92093-0603
Email: nbroadbent <@t> ucsd.edu
Phone: (858) 642 3628 or
(858) 552 8585 x 7853
Fax: (858) 552 7457
------------------------------
Message: 2
Date: Thu, 06 Dec 2007 13:33:35 -0500
From: "Mirela Hasu" <mhasu <@t> ottawaheart.ca>
Subject: [Histonet] immunohitochem for IFN gamma
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s757fa4a.030 <@t> mail.ottawaheart.ca>
Content-Type: text/plain; charset=US-ASCII
Hello,
Does anybody have performed the immunohistochemistry for Interferon
gamma on mice heart tissue and on aortic lesions.
Would be very good if I can get a protocol that worked well.
Thank you,
Mirela
------------------------------
Message: 3
Date: Thu, 6 Dec 2007 13:34:00 -0500
From: Philip Oshel <oshel1pe <@t> cmich.edu>
Subject: Re: [Histonet] reticulin/snooks
To: Histonet <@t> Pathology.swmed.edu
Message-ID: <f06240808c37dee1e177d@[141.209.160.249]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
Cheri,
The electron microscopy supply companies are the usual places for
uranyl salts. These are Biological Stain Commission certified, but
they are the pure (or nearly) powders.
Electron Microscopy Supplies
http://www.emsdiasum.com/microscopy/products/chemicals/tannic.aspx
Structure Probe, Inc. aka SPI
http://www.2spi.com/catalog/chem/stain.shtml
Also, regular chemical companies like Sigma-Aldrich have uranyl nitrate.
http://www.sigmaaldrich.com/catalog/search/SearchResultsPage/PricingAvailability
/FLUKA;94270
Phil
>Who uses Snook's method for their retic stain?? If you do where do you get
>your uranium nitrate?? I ask Rowley and they don't supply it anymore because
>of it's hazards?? Am I missing something???
>
>Cheri Miller HT ASCP
>
>Histology Supervisor
>Physicians Laboratory Services, Inc.
>
>Omaha, NE 68117
>
>402 738 5052
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576
------------------------------
Message: 4
Date: Thu, 6 Dec 2007 11:27:02 -0800
From: "Martin, Gary" <gmartin <@t> marshallmedical.org>
Subject: [Histonet] Patchy staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6ED9D4252F278841A0593D3D788AF24C0152E46B <@t> mailsvr.MARSHMED.local>
Content-Type: text/plain; charset="us-ascii"
Thank you to all who responded to my request for help concerning the
voids/patchy staining. It seems as though we were delinquent in
changing our reagents ... that solved the problem. The wet noodle has
been activated :)
Much thanks .
Gary
------------------------------
Message: 5
Date: Thu, 6 Dec 2007 11:35:06 -0800
From: "Nicola J. Broadbent Ph.D" <nbroadbent <@t> ucsd.edu>
Subject: [Histonet] Re:Interrupted thionin staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <003a01c8383f$1bc1cd00$8a4cef84 <@t> Nicola>
Content-Type: text/plain; charset="us-ascii"
Thank you to everyone who responded to my request regarding thionin staining
and zylene. I appreciate all the advice! The staining worked out great!
Nicola
Nicola J. Broadbent Ph.D,
Asst. Project Scientist
Department of Psychiatry 0603,
UCSD School of Medicine,
9500 Gilman Dr,
La Jolla, CA, 92093-0603
Email: nbroadbent <@t> ucsd.edu
Phone: (858) 642 3628 or
(858) 552 8585 x 7853
Fax: (858) 552 7457
------------------------------
Message: 6
Date: Thu, 6 Dec 2007 15:07:32 -0500
From: "Fail, Mildred M." <failm <@t> musc.edu>
Subject: RE: [Histonet] Re: Slide quality
To: "Kemlo Rogerson" <Kemlo.Rogerson <@t> waht.swest.nhs.uk>, "Robert
Richmond" <RSRICHMOND <@t> aol.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E6A89143ADFAEE4490AFB7DD7E4CEEA712CC7A <@t> EVS2.clinlan.local>
Content-Type: text/plain; charset="US-ASCII"
More emphasis is placed on quantity rather than quality. Here the main
histology lab is responsible for cutting everthing but controls for
IHC/SS. Attempts to catch bad slides before staining were met with
contempt, even though my superior had directed me to check the slides.
Quite frankly I had to stop checking the slides, it just created too
much animosity.
Rena Fail
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kemlo
Rogerson
Sent: Thursday, December 06, 2007 4:59 AM
To: Robert Richmond; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Slide quality
You couldn't get the average pathologist, definitely including this one,
to even notice most of the folds and wrinkles that occur in sections.
We've become so used to ignoring them that when we teach
photomicrography to residents, we have to remind them not to photograph
areas in the slide with wrinkles in them - they look like hell when
they're projected.
I think that asking the pathologist to document wrinkles and folds is
unreasonable.
In the various labs I do pathology in, the recurrent problem is GI
biopsies with shatter and "window-blind" artifact. My requests to
address the problem are usually ignored. Very few pathology services do
separate processor runs for small specimens, and I've never been able to
get a laboratory to even consider it.
If I ran the zoo, I'd have a double headed microscope (not permitted for
pathologists in small pathology services), and I'd look at the day's run
of slides with a senior histotechnologist nearly every day. That to my
mind might launch an effective quality assurance program.
I agree with you (before you argue with me!) that most pathologists in
this circumstance would be so abusive that the exercise would be quite
unendurable for the technologist.
Robert
I'm surprised that you don't look at the slides with the Tech; how does
he/ she know if they are doing a good job. In my experience when my
Pathologist was kind enough to teach me some smatterings of
Dermatopathology it was stunningly powerful in showing me the error of
my ways. I don't understand why Techs/ BMSs don't look at slides before
they go to the Pathologist and even take a crack at the diagnosis. On
one or two occasions I remember I've spotted CIN3 on a Lletz that wasn't
spotted by the Pathologist but then I was a Cytologist; wouldn't be any
good at anything non- cervical; but I could learn!!!
Kemlo Rogerson
Pathology Manager
DD 01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
The road to success is dotted with many tempting parking places.
--Author Unknown
This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful.
Please inform me that this message has gone astray before deleting it.
Thank you for your co-operation
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Thu, 06 Dec 2007 15:21:59 -0500
From: "Janice Mitchell" <MITCHELLJA <@t> email.chop.edu>
Subject: [Histonet] What is the CAP required water quality for a
histology lab? We have been told we need to test our D
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s75813a5.052 <@t> email.chop.edu>
Content-Type: text/plain; charset=US-ASCII
What is the CAP required water quality for a histology lab? We have
been told we need to test our DI water monthly but no one seems to know
what quality level our water should be. Thanks, Janice
------------------------------
Message: 8
Date: Fri, 7 Dec 2007 08:05:21 +1100
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Re: Slide quality
To: "Kemlo Rogerson" <Kemlo.Rogerson <@t> waht.swest.nhs.uk>, "Robert
Richmond" <RSRICHMOND <@t> aol.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC555202FAFD95 <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"
A good histotechnologists must be able to recognise tissue types. They
must know when a section is adequate (nay should I say perfect?) for
diagnosis. In my laboratories, my staff hate it when a medico tells them
that a section is inadequate. Hey we know more about the science of
Histopathology than a medico, that's why we are scientists or
technicians.
Granted some things slip through since we can only check a sample of the
days work but without basic histology knowledge how can one
differentiate a trichrome, or elastc stain?
Solving problems is our job. Take the GI Bx problem. We have used a
Lieca caroussel-type processor for many years to process these. It is a
lot gentler on the tissue but for placenta and large thick tissue blocks
we use a Shandon enclosed processor (more umph!).
Train your staff to recognise good sections, give them spot quizzes on
unknown tissues. More importantly when a problem is noted and you know
how to solve it, don't. Let your staff knuckle it out.
Eg, the eosin staining tray in the automatic stainer was low and only
part of the section stained, yet the previous run with a full rack of
slides was OK. Why? You will be surprised at some of the answers. Here
is where you show your staff how to solve the problems. It gets them
thinking. It provides a work environment that is interesting since to
learn something new everyday, especially when they don't realise they
are doing it, really can induce a productive and responsive workforce.
Interesting I even wonder at some of our own ?experts in our External
QAP. An assessment of a Perl's stain (stained in a coplin jar, not on a
rack) stated that staining was uneven across the section. On review, it
was noted that the slide stained (supplied by the QAP) was of uneven
thickness. Couldn't the assessors pick this up? Anyway enough flamming
before I get into real trouble!!
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kemlo
Rogerson
Sent: Thursday, 6 December 2007 8:59 PM
To: Robert Richmond; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Slide quality
You couldn't get the average pathologist, definitely including this one,
to even notice most of the folds and wrinkles that occur in sections.
We've become so used to ignoring them that when we teach
photomicrography to residents, we have to remind them not to photograph
areas in the slide with wrinkles in them - they look like hell when
they're projected.
I think that asking the pathologist to document wrinkles and folds is
unreasonable.
In the various labs I do pathology in, the recurrent problem is GI
biopsies with shatter and "window-blind" artifact. My requests to
address the problem are usually ignored. Very few pathology services do
separate processor runs for small specimens, and I've never been able to
get a laboratory to even consider it.
If I ran the zoo, I'd have a double headed microscope (not permitted for
pathologists in small pathology services), and I'd look at the day's run
of slides with a senior histotechnologist nearly every day. That to my
mind might launch an effective quality assurance program.
I agree with you (before you argue with me!) that most pathologists in
this circumstance would be so abusive that the exercise would be quite
unendurable for the technologist. Robert
I'm surprised that you don't look at the slides with the Tech; how does
he/ she know if they are doing a good job. In my experience when my
Pathologist was kind enough to teach me some smatterings of
Dermatopathology it was stunningly powerful in showing me the error of
my ways. I don't understand why Techs/ BMSs don't look at slides before
they go to the Pathologist and even take a crack at the diagnosis. On
one or two occasions I remember I've spotted CIN3 on a Lletz that wasn't
spotted by the Pathologist but then I was a Cytologist; wouldn't be any
good at anything non- cervical; but I could learn!!!
Kemlo Rogerson
Pathology Manager
DD 01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
The road to success is dotted with many tempting parking places.
--Author Unknown
This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful.
Please inform me that this message has gone astray before deleting it.
Thank you for your co-operation
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 9
Date: Thu, 6 Dec 2007 17:42:21 -0500
From: Sharon <scoop <@t> mail.nih.gov>
Subject: [Histonet] Nissl staining question: ignorant question from an
ignorant person
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <p0602040cc37e2444f563@[156.40.160.151]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
Dear Histonetters,
I have some questions about Nissl staining - (I have looked these
issues up in histology texts but am still confused because I have
received contradictory information - please clarify)
Here are my ridiculous questions:
1. Is crystal violet ever used to stain epon embedded tissue for
pathology? (I don't think so)
2. Is toluidine blue used as a Nissl stain? (I think so)
3. Are there advantages to Nissl staining with Cresyl Violet vs.
Toluidine blue? (when you are just looking for pathology with no
other stain - eg. not using the Toluidine blue or Cresyl Violet as a
counter stain for some other stain).
4. Is Cresyl Violet used for anything other than a Nissl stain in brain?
Thanks for your indulgence.
Sharon
------------------------------
Message: 10
Date: Thu, 6 Dec 2007 17:26:23 -0800
From: "Bob Nienhuis" <bob.nienhuis <@t> gmail.com>
Subject: [Histonet] Golgi slide bubbles
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<45109da50712061726t4434605ak1396208e3039d7 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Doing Golgi-Cox staining of mouse brains and am having a problem with
bubbles appearing under the coverslip after va few days. I'm
guessing it's due to the solvent evaporating from the thick mounting
medium. Sections were cut frozen using the Kolb and McClimans (1986)
cryostat technique. 100 micron sections, DPX mounting medium.
Any suggestions?
Bob Nienhuis
------------------------------
Message: 11
Date: Thu, 6 Dec 2007 21:57:59 -0600
From: "Joe Nocito" <jnocito <@t> satx.rr.com>
Subject: Re: [Histonet] What is the CAP required water quality for a
histology lab? We have been told we need to test our D
To: "Janice Mitchell" <MITCHELLJA <@t> email.chop.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <005201c83885$5bf33350$0302a8c0 <@t> yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Janice,
as luck would have it, I just happen to have the new checklist in front of
me (no, really, I do)
CAP revised this requirement on the new 9/27/07 edition
GEN.41500
Clinical Laboratory Reagent Water (CLRW) is the old Type I water which CAP
has graciously enclosed in the checklist.
Special Reagent Water (SRW) is now defined by laboratory procedures that
need different specifications than CLRW, but CAP does not graciously enclose
that requirement
Instrument Feed Water (IFW) is determined by the equipment manufacturer.
They reference the CLSI Guideline for information on special reagent water,
whatever that means.
Glad I could clear that up for you. No need to thank me, really.
Now, I have to go find the CLSI Guideline myself.
And people wonder why I have a problem with CAP. They couldn't leave well
enough alone, could they?
JTT
----- Original Message -----
From: "Janice Mitchell" <MITCHELLJA <@t> email.chop.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, December 06, 2007 2:21 PM
Subject: [Histonet] What is the CAP required water quality for a histology
lab? We have been told we need to test our D
> What is the CAP required water quality for a histology lab? We have
> been told we need to test our DI water monthly but no one seems to know
> what quality level our water should be. Thanks, Janice
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Fri, 7 Dec 2007 07:00:33 +0200
From: Dalia El Rouby <daliaelrouby <@t> hotmail.com>
Subject: [Histonet] bromephenol blue
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU122-W35DBD08E2F1522A03AAF1D4680 <@t> phx.gbl>
Content-Type: text/plain; charset="windows-1256"
DEar Collegues:
i am in urgent need for the detailed procedure for bromephenol blue stain used
to detect protein. waiting for your kind reply
_________________________________________________________________
News, entertainment and everything you care about at Live.com. Get it now!
http://www.live.com/getstarted.aspx
------------------------------
Message: 13
Date: Fri, 7 Dec 2007 15:17:43 +0200
From: "Sofia Havaki" <shavaki <@t> med.uoa.gr>
Subject: [Histonet] CK8 immunofluorescence problem
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <00f301c838d3$8d6f0fa0$817386c3 <@t> Tom>
Content-Type: text/plain; charset="iso-8859-7"
Dear Histonetters,
I applied immunofluorescence on primary cultures of breast cancer cells and on
cells from the established cell line MDA-MB-231 for the localization of
cytokeratin 8, using the primary monoclonal antibody human anti-cytokeratin 8,
purchased from Sigma (code No: C5301). My problem is that the immunofluorescence
protocol that I followed, gave perfect results in the breast cancer cells of the
culture cell line, while in the cells of the primary culture it didn't work (the
immunofluorescence was very weak and obscure).
The cells are permeabilized with Triton X-100 0.5 % diluted in PBS for 5min.
Could the cells of the primary culture need different permeabilization time
compared with those of the cell line?
Could someone help??
Thank you in advance
Sophia Havaki
------------------------------
Message: 14
Date: Fri, 7 Dec 2007 14:32:22 +0100 (CET)
From: "Valeria Berno" <valeria.berno <@t> embl.it>
Subject: [Histonet] c-fos
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1806.10.251.1.146.1197034342.squirrel <@t> 10.251.1.146>
Content-Type: text/plain;charset=iso-8859-1
Hi there,
one of the user I am working with is having tough time to found a good
antibody for c-fos to use in cryosection of mouse brain (30micron size) in
immunofluorescence.
Any suggestion? should she use a different protocol?The protocol right now
is perfusion with PFA, sucrose, OCT,cutting, permebilization with
0.3%triton, blocking with serum and secondary A488.
thanks in advance
Valeria
------------------------------
Message: 15
Date: Fri, 7 Dec 2007 10:00:05 -0500
From: "Smith, Allen" <asmith <@t> mail.barry.edu>
Subject: RE: [Histonet] Nissl staining question: ignorant question
from an ignorant person
To: 'Sharon' <scoop <@t> mail.nih.gov>
Cc: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E4132130AC2F764D8C173C5400D53042586FE1291D <@t> exchsrv02.barrynet.barry.edu>
Content-Type: text/plain; charset="us-ascii"
My cytology professor, Seymour Gelfant, recommended toluidine blue as a Nissl
stain. Lillie's HISTOPATHOLOGIC TECHNIC also says that toluidine blue can be
used as a Nissl stain.
Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon
Sent: Thursday, December 06, 2007 5:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Nissl staining question: ignorant question from an ignorant
person
Dear Histonetters,
I have some questions about Nissl staining - (I have looked these
issues up in histology texts but am still confused because I have
received contradictory information - please clarify)
Here are my ridiculous questions:
1. Is crystal violet ever used to stain epon embedded tissue for
pathology? (I don't think so)
2. Is toluidine blue used as a Nissl stain? (I think so)
3. Are there advantages to Nissl staining with Cresyl Violet vs.
Toluidine blue? (when you are just looking for pathology with no
other stain - eg. not using the Toluidine blue or Cresyl Violet as a
counter stain for some other stain).
4. Is Cresyl Violet used for anything other than a Nissl stain in brain?
Thanks for your indulgence.
Sharon
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 49, Issue 9
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