[Histonet] Nissl staining question: ignorant question from an
ignorant person
Smith, Allen
asmith <@t> mail.barry.edu
Fri Dec 7 09:00:05 CST 2007
My cytology professor, Seymour Gelfant, recommended toluidine blue as a Nissl stain. Lillie's HISTOPATHOLOGIC TECHNIC also says that toluidine blue can be used as a Nissl stain.
Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon
Sent: Thursday, December 06, 2007 5:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Nissl staining question: ignorant question from an ignorant person
Dear Histonetters,
I have some questions about Nissl staining - (I have looked these
issues up in histology texts but am still confused because I have
received contradictory information - please clarify)
Here are my ridiculous questions:
1. Is crystal violet ever used to stain epon embedded tissue for
pathology? (I don't think so)
2. Is toluidine blue used as a Nissl stain? (I think so)
3. Are there advantages to Nissl staining with Cresyl Violet vs.
Toluidine blue? (when you are just looking for pathology with no
other stain - eg. not using the Toluidine blue or Cresyl Violet as a
counter stain for some other stain).
4. Is Cresyl Violet used for anything other than a Nissl stain in brain?
Thanks for your indulgence.
Sharon
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