[Histonet] c-fos

Valeria Berno valeria.berno <@t> embl.it
Fri Dec 7 07:32:22 CST 2007

Hi there,

one of the user I am working with is having tough time to found a good
antibody for c-fos to use in cryosection of mouse brain (30micron size) in

Any suggestion? should she use a different protocol?The protocol right now
is perfusion with PFA, sucrose, OCT,cutting, permebilization with
0.3%triton, blocking with serum and secondary A488.

thanks in advance


More information about the Histonet mailing list