[Histonet] RE: Florida licensure question
Meilus, Sheri D.
Sheri.Meilus <@t> va.gov
Tue Dec 4 10:10:00 CST 2007
Anderson Continuing Education offers a 2 hour course on Medical Errors. It's called Prevention of Medical Errors 2007. Their web site is www.andersonCE.com They are recognized by Florida as a provider for CEUS. Relatively inexpensive too.
S
Sheri Meilus, HT(ASCP)QIHC
Anatomic Pathology Supervisor
Bay Pines VAMC Bay Pines, FL 33744
727-398-6661 4596
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, December 04, 2007 8:53 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 49, Issue 4
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Today's Topics:
1. Florida licensure question (Beth Cox)
2. cardboard storage boxes (Orr, Rebecca)
3. re:Problems in Neurofilaments-31 Staining (Carl Hobbs)
4. SMI-31 phosphorylated neurofilament (Houston, Ronald)
5. RE: RE: Retic jargon (Monson, Frederick )
6. RE: RE: Retic jargon - Wiki (Monson, Frederick )
7. CD26 (Tarango, Mark)
8. H Pylori (Mitchell, Jeannette M.)
9. Re: H Pylori (Rene J Buesa)
10. Re: Florida licensure question (Rene J Buesa)
11. Re: Florida licensure question (JMeade0710 <@t> aol.com)
12. Re: Florida licensure question (Rene J Buesa)
13. Schiff's reagent (Taben Hale)
14. Re: Schiff's reagent (Rene J Buesa)
15. uranium - question for microscope slide manufacturers
possibly (Andrea Grantham)
16. Re: Schiff's reagent (Andrea Grantham)
17. RE: Schiff's reagent (Cheryl R. Kerry)
18. massons trichrome (kamal prasad)
19. Permanent Red stain (Young Kwun)
20. Re: uranium - question for microscope slide manufacturers
possibly (louise renton)
21. RE: Schiff's reagent (Kemlo Rogerson)
22. Re: Florida licensure question (Dawn Cowie)
23. Re: uranium - question for microscope slide manufacturers
possibly (Rene J Buesa)
24. Re: Permanent Red stain (Rene J Buesa)
25. RE: H Pylori (Dawson, Glen)
----------------------------------------------------------------------
Message: 1
Date: Mon, 03 Dec 2007 13:14:35 -0500
From: Beth Cox <bethcoxx <@t> gmail.com>
Subject: [Histonet] Florida licensure question
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4754478B.6010407 <@t> gmail.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
I work as a traveling tech, and do temporary assignments all over the
country. I want to apply for my Florida license, but I need to fulfill
that requirement for CE credits in "medical error prevention". Do any
of you Florida people have suggestions on how/where I can get that
without too much hassle?? Maybe something online? Thanks,
Beth
------------------------------
Message: 2
Date: Mon, 3 Dec 2007 13:17:59 -0600
From: "Orr, Rebecca" <ROrr <@t> enh.org>
Subject: [Histonet] cardboard storage boxes
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DB61DED572F83344AE6894373372486B977215 <@t> EXCHANGE03.enhnet.org>
Content-Type: text/plain; charset="us-ascii"
Hi Everyone,
I am in search of a specific type of storage box.
It's a cardboard box that holds 100 scintillation bottles. Square 12" x
12" x 2.5" H
I don't need the bottles; I just need the box that has the 100 spaced
dividers (empty box)
I have asked help from the manufacturer of the bottles as well as the
actual box supplier, alas, to no avail.
If anyone has this type of box I would be very appreciative and would
remember you fondly in my last will and testament.
Please let me know and I'll arrange to have it sent to me. And don't
ask me why I need it, it's a long long boring story.
Thanks
Bec
Becky Orr CLA,HT(ASCP)QIHC
Anatomic Pathology
Evanston Northwestern Healthcare
847-570-2771
------------------------------
Message: 3
Date: Mon, 3 Dec 2007 20:13:17 -0000
From: "Carl Hobbs" <carl.hobbs <@t> kcl.ac.uk>
Subject: [Histonet] re:Problems in Neurofilaments-31 Staining
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <005701c835e8$f1b00a70$4101a8c0 <@t> carlba65530bda>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Hi.
If you want to demonstrate NFs in general, I am surprised that you have
chosen to test an Ab reagent that has not been validated for pwax sections,
by Abcam.
It may well be that this SMI31 clone is not pwax-reactive.
If I am wrong..my apologies.
Sigma's anti NF 68 (N-5139), NF160 ( N-5264), NF200 ( N-0142) alone or in a
cocktail are very good.
Also, "RMO" clone (NF160) and RT97 clone ( NF200) are superb Ab reagents.
Imho, these are the "standards" for Neurofilament detection.
Carl
------------------------------
Message: 4
Date: Mon, 3 Dec 2007 15:31:05 -0500
From: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
Subject: [Histonet] SMI-31 phosphorylated neurofilament
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<979FF5962E234F45B06CF0DB7C1AABB213B9CAE6 <@t> chi2k3ms01.columbuschildrens.net>
Content-Type: text/plain; charset="us-ascii"
Sorry deleted original message so do not know who posted the query but
use the antibody from Covance, cat # SMI-31R; excellent results with
1:800 dilution after EDTA retrieval.
No experience with this antibody from AbCam but have had good results
with other antibodies from them.
http://store.crpinc.com/pdfdatasheet.aspx?catalogno=SMI-31R
Ronnie Houston, MS, HT(ASCP)QIHC
Anatomic Pathology Manager
Nationwide Children's Hospital
700 Children's Drive
Columbus, OH 43205
(614) 722 5465
Ronald.Houston <@t> NationwideChildrens.org
Columbus Children's Hospital is now Nationwide Children's Hospital
www.NationwideChildrens.org <http://www.nationwidechildrens.org/>
------------------------------
Message: 5
Date: Mon, 3 Dec 2007 15:38:31 -0500
From: "Monson, Frederick " <FMonson <@t> wcupa.edu>
Subject: RE: [Histonet] RE: Retic jargon
To: "Renko, Heather D." <Heather.D.Renko <@t> osfhealthcare.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<641CEFFC7E5B6C42AB59539653FD08230517A42F <@t> wcu-ex-emp2.PASSHE.LCL>
Content-Type: text/plain; charset="us-ascii"
Maresch (1905) was an old dead guy when I was a young guy in 1965 and
working with the "Silver Impregnation for Retiuclin" - by Pearse (1968)
out of Maresch? Book is gone, and I'm just lingering.
Two historic points.
1. The above impregnation - of only meager vicarious value -
'showed' fine fibers/fibrils around cells (especially in hematopoietic
tissue) that were generally unstained by other prevailing methods, AND
did NOT 'color' collagen except to a 'light tan'.
2. Even though Watson and Crick presented the model for DNA in
1953, it did not inhabit many undergraduate classrooms until the
mid-1960's - acknowledging exceptions such as Havahd and Yail.
Thus, even though silver impregnation of reticular fibers, reticulin or
a reticulum is still performed, the procedure does not beat the MAb for
Collagen III. Although, I must add, a notable MAb for an (human)
elastin epitope proved conclusively (and negatively!) in the mid 1990's
that the rabbit lacked elastin in its urinary bladder despite proof to
the contrary from a widely accepted Gomori's Aldehyde Fuchsin stain.
I still say, if we just stay focused on the significant surgical
importance of dissection vs. disection, we will be far better off in the
long run. We absolutely must dissuade young medical students from
either enunciating or doing dIsections.
In the domain of the physical sciences, we must pass a law that prevents
anyone who can't pronounce the word 'NUCLEAR', from ever purchasing or
having access to a red telephone.
Cheers,
Fred Monson
Frederick C. Monson, PhD
Technical Director
Microanalysis and Imaging Research and Training Center (MIRTC)
Large Scientific Instrument Core
Geology, West Chester University
S. Church St. and W. Rosedale Ave.
West Chester, PA, 19320
610-738-0437
fmonson <@t> wcupa.edu
New Scheduler: http://lexspiac.wcupa.edu/cgi-bin/ureserve_gold.pl
Web Page: http://lexspiac.wcupa.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Renko,
Heather D.
Sent: Friday, November 30, 2007 1:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Retic jargon
We say "Reticulum" ???
The term reticulin was coined in 1892 by M. Siegfried.[2]
<http://en.wikipedia.org/wiki/>
Today the term reticulin or reticular fiber is restricted to fibers
composed of type III collagen <http://en.wikipedia.org/wiki/Collagen> .
However, during the pre-molecular era, there was confusion in the use of
the term 'reticulin', which was used to describe two structures:
* the argyrophilic (silver staining) fibrous structures present in
basement membranes <http://en.wikipedia.org/wiki/Basement_membrane>
* histologically similar fibers present in developing connective
tissue[3] <http://en.wikipedia.org/wiki/> .
The history of the reticulin silver stain is reviewed by Puchtler et al.
(1978).[4] <http://en.wikipedia.org/wiki/> The abstract of this paper
says:
Maresch (1905) introduced Bielschowsky's silver
impregnation technic for neurofibrils as a stain for reticulum fibers,
but emphasized the nonspecificity of such procedures. This lack of
specificity has been confirmed repeatedly. Yet, since the 1920's the
definition of "reticulin" and studies of its distribution were based
solely on silver impregnation technics. The chemical mechanism and
specificity of this group of stains is obscure. Application of Gomori's
and Wilder's methods to human tissues showed variations of staining
patterns with the fixatives and technics employed. Besides reticulum
fibers, various other tissue structures, e.g. I bands of striated
muscle, fibers in nervous tissues, and model substances, e.g.
polysaccharides, egg white, gliadin, were also stained. Deposition of
silver compounds on reticulum fibers was limited to an easily removable
substance; the remaining collagen component did not bind silver. These
histochemical studies indicate that silver impregnation technics for
reticulum fibers have no chemical significance and cannot be considered
as histochemical technics for "reticulin" or type III collagen.
Who'd a thunk it? TGIF
Heather D. Renko, Histology Coordinator
OSF Saint Anthony Medical Center
5666 East State Street
Rockford, Illinois 61108
Direct: 815-395-5410
Heather.D.Renko <@t> osfhealthcare.org
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Message: 6
Date: Mon, 3 Dec 2007 15:39:50 -0500
From: "Monson, Frederick " <FMonson <@t> wcupa.edu>
Subject: RE: [Histonet] RE: Retic jargon - Wiki
To: "Renko, Heather D." <Heather.D.Renko <@t> osfhealthcare.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<641CEFFC7E5B6C42AB59539653FD08230517A431 <@t> wcu-ex-emp2.PASSHE.LCL>
Content-Type: text/plain; charset="us-ascii"
Further, if we don't know more than is given on Wikipedia, we deserve to
believe what is written there as Gospel.
Frederick C. Monson, PhD
Technical Director
Microanalysis and Imaging Research and Training Center (MIRTC)
Large Scientific Instrument Core
Geology, West Chester University
S. Church St. and W. Rosedale Ave.
West Chester, PA, 19320
610-738-0437
fmonson <@t> wcupa.edu
New Scheduler: http://lexspiac.wcupa.edu/cgi-bin/ureserve_gold.pl
Web Page: http://lexspiac.wcupa.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Renko,
Heather D.
Sent: Friday, November 30, 2007 1:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Retic jargon
We say "Reticulum" ???
The term reticulin was coined in 1892 by M. Siegfried.[2]
<http://en.wikipedia.org/wiki/>
Today the term reticulin or reticular fiber is restricted to fibers
composed of type III collagen <http://en.wikipedia.org/wiki/Collagen> .
However, during the pre-molecular era, there was confusion in the use of
the term 'reticulin', which was used to describe two structures:
* the argyrophilic (silver staining) fibrous structures present in
basement membranes <http://en.wikipedia.org/wiki/Basement_membrane>
* histologically similar fibers present in developing connective
tissue[3] <http://en.wikipedia.org/wiki/> .
The history of the reticulin silver stain is reviewed by Puchtler et al.
(1978).[4] <http://en.wikipedia.org/wiki/> The abstract of this paper
says:
Maresch (1905) introduced Bielschowsky's silver
impregnation technic for neurofibrils as a stain for reticulum fibers,
but emphasized the nonspecificity of such procedures. This lack of
specificity has been confirmed repeatedly. Yet, since the 1920's the
definition of "reticulin" and studies of its distribution were based
solely on silver impregnation technics. The chemical mechanism and
specificity of this group of stains is obscure. Application of Gomori's
and Wilder's methods to human tissues showed variations of staining
patterns with the fixatives and technics employed. Besides reticulum
fibers, various other tissue structures, e.g. I bands of striated
muscle, fibers in nervous tissues, and model substances, e.g.
polysaccharides, egg white, gliadin, were also stained. Deposition of
silver compounds on reticulum fibers was limited to an easily removable
substance; the remaining collagen component did not bind silver. These
histochemical studies indicate that silver impregnation technics for
reticulum fibers have no chemical significance and cannot be considered
as histochemical technics for "reticulin" or type III collagen.
Who'd a thunk it? TGIF
Heather D. Renko, Histology Coordinator
OSF Saint Anthony Medical Center
5666 East State Street
Rockford, Illinois 61108
Direct: 815-395-5410
Heather.D.Renko <@t> osfhealthcare.org
========================================================================
======
The information in this message is confidential and may be legally
privileged. Access to this message by anyone other than the addressee is
not authorized. If you are not the intended recipient, or an agent of
the intended recipient, any disclosure, copying, or distribution of the
message or any action or omission taken by you in reliance on it, is
prohibited and may be unlawful. If you have received this message in
error, please contact the sender immediately and permanently delete the
original e-mail, attachment(s), and any copies.
========================================================================
======
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------------------------------
Message: 7
Date: Mon, 3 Dec 2007 13:11:28 -0800
From: "Tarango, Mark" <mtarango <@t> nvcancer.org>
Subject: [Histonet] CD26
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<5AEC610C1CE02945BD63A395BA763EDE011B7001 <@t> NVCIEXCH02.NVCI.org>
Content-Type: text/plain; charset=us-ascii
Has anyone ever stained for CD26 in FFPE human tissue? If so, please
share your experiences. I'm supposed to get this stain up and running
for a clinical trial that is starting up and I'm not having much luck.
The Japanese group we're working with is coming to town in a few weeks
and I'm getting a little nervous.
I need to stain in kidney and some other normal tissues, but I want to
see it in tonsil first so I can really have some faith in the stain. I
have several antibodies, including the humanized version that is the
drug. The humanized antibody is biotinylated.
Thanks,
Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV 89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663
"EMF <nvcancer.org>" made the following annotations.
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Message: 8
Date: Mon, 3 Dec 2007 16:26:50 -0500
From: "Mitchell, Jeannette M." <Jeannette.Mitchell <@t> vtmednet.org>
Subject: [Histonet] H Pylori
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DFD774BB7BA21545914AD7765EC3BC744F72562E <@t> EMAIL1.fahc.fletcherallen.org>
Content-Type: text/plain; charset="us-ascii"
Has anyone ever had dirty staining in negative gastric comtrol? I am getting some nonspecific staining in some of my gastric biopsies. The nonspecific staining I am talking about is groups of ppt., almost looks like bacteria but is way to big and is not uniform in size or clumps. I don't see it in any of the other Ipexes that we are currently performing just the gastric biopsies. Any ideas anyone??
Jeannette Mitchell, BS, HTL(ASCP), QIHC
R&D Histotechnologist
EP1-119
Fletcher Allen Health Care
Burlington, VT 05403
------------------------------
Message: 9
Date: Mon, 3 Dec 2007 13:32:28 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] H Pylori
To: "Mitchell, Jeannette M." <Jeannette.Mitchell <@t> vtmednet.org>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <601152.81805.qm <@t> web61217.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
If you are using silver (Steiner) it can be the silver solution and the developer.
René J.
"Mitchell, Jeannette M." <Jeannette.Mitchell <@t> vtmednet.org> wrote:
Has anyone ever had dirty staining in negative gastric comtrol? I am getting some nonspecific staining in some of my gastric biopsies. The nonspecific staining I am talking about is groups of ppt., almost looks like bacteria but is way to big and is not uniform in size or clumps. I don't see it in any of the other Ipexes that we are currently performing just the gastric biopsies. Any ideas anyone??
Jeannette Mitchell, BS, HTL(ASCP), QIHC
R&D Histotechnologist
EP1-119
Fletcher Allen Health Care
Burlington, VT 05403
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Message: 10
Date: Mon, 3 Dec 2007 13:33:27 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Florida licensure question
To: Beth Cox <bethcoxx <@t> gmail.com>, histonet <@t> lists.utsouthwestern.edu
Message-ID: <280640.93519.qm <@t> web61223.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
E-mail the Florida Histotechnology Society.
They are very helpful.
René J.
Beth Cox <bethcoxx <@t> gmail.com> wrote:
I work as a traveling tech, and do temporary assignments all over the
country. I want to apply for my Florida license, but I need to fulfill
that requirement for CE credits in "medical error prevention". Do any
of you Florida people have suggestions on how/where I can get that
without too much hassle?? Maybe something online? Thanks,
Beth
_______________________________________________
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Message: 11
Date: Mon, 3 Dec 2007 17:02:17 EST
From: JMeade0710 <@t> aol.com
Subject: Re: [Histonet] Florida licensure question
To: bethcoxx <@t> gmail.com, histonet <@t> lists.utsouthwestern.edu
Message-ID: <bd6.20e48fe3.3485d6e9 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
I too am a traveler, and applying for the Florida license and in the same
position that Beth is in. Any help would be appreciated.
Jerry Meade
In a message dated 12/3/2007 1:15:17 P.M. Eastern Standard Time,
bethcoxx <@t> gmail.com writes:
I work as a traveling tech, and do temporary assignments all over the
country. I want to apply for my Florida license, but I need to fulfill
that requirement for CE credits in "medical error prevention". Do any
of you Florida people have suggestions on how/where I can get that
without too much hassle?? Maybe something online? Thanks,
Beth
_______________________________________________
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Message: 12
Date: Mon, 3 Dec 2007 14:23:26 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Florida licensure question
To: JMeade0710 <@t> aol.com, bethcoxx <@t> gmail.com,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <778435.73033.qm <@t> web61212.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Same advise, e-mail the Florida Society for Histotechnologist.
René J.
JMeade0710 <@t> aol.com wrote:
I too am a traveler, and applying for the Florida license and in the same
position that Beth is in. Any help would be appreciated.
Jerry Meade
In a message dated 12/3/2007 1:15:17 P.M. Eastern Standard Time,
bethcoxx <@t> gmail.com writes:
I work as a traveling tech, and do temporary assignments all over the
country. I want to apply for my Florida license, but I need to fulfill
that requirement for CE credits in "medical error prevention". Do any
of you Florida people have suggestions on how/where I can get that
without too much hassle?? Maybe something online? Thanks,
Beth
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
**************************************Check out AOL's list of 2007's hottest
products.
(http://money.aol.com/special/hot-products-2007?NCID=aoltop00030000000001)
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Message: 13
Date: Mon, 3 Dec 2007 17:25:55 -0500
From: "Taben Hale" <taben.hale <@t> gmail.com>
Subject: [Histonet] Schiff's reagent
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<dffe18930712031425q75de98f8t7398db525cdc584b <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
What is the shelf-life and stability of Schiff's reagent? Can it be used
multiple times?
--
Taben M Hale, PhD
Postdoctoral Fellow
Universite de Montreal
Dept Pharmacologie
(514)343-6111 ext. 4968
------------------------------
Message: 14
Date: Mon, 3 Dec 2007 14:29:41 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Schiff's reagent
To: Taben Hale <taben.hale <@t> gmail.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <453373.58567.qm <@t> web61216.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
It has to be kept in the refrigerator, and yes, it can be used several times.
You can test how is working by adding a few drops of the Schiff's reagent to NBF, it it reacts quickly with a magenta color, is working OK, if not, discard it.
René J.
Taben Hale <taben.hale <@t> gmail.com> wrote:
What is the shelf-life and stability of Schiff's reagent? Can it be used
multiple times?
--
Taben M Hale, PhD
Postdoctoral Fellow
Universite de Montreal
Dept Pharmacologie
(514)343-6111 ext. 4968
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Message: 15
Date: Mon, 03 Dec 2007 15:50:16 -0700
From: Andrea Grantham <algranth <@t> u.arizona.edu>
Subject: [Histonet] uranium - question for microscope slide
manufacturers possibly
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.2.3.4.1.20071203153611.01ef6358 <@t> algranth.inbox.email.arizona.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
I have a question about slides containing concentrations of uranium.
One of my investigators is working on a project where they are
lasering tissue off of slides and checking it for the amount of
uranium in the tissue. The laser actually vaporizes the tissue and it
goes into a machine where it is tested for uranium content. They were
getting some strange readings and tested the slides alone without
tissue and found that the slides alone had a pretty high uranium content.
So she is asking - Is there some coating to put on the slides to
block the uranium?
My question is this - is there really uranium in slides and just how
much uranium is in a glass slide? Can this be dangerous?
Are there some slides out there that do not contain uranium?
I hope somebody has an answer for my weird question of the day.
Thanks.
Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html
------------------------------
Message: 16
Date: Mon, 03 Dec 2007 15:55:01 -0700
From: Andrea Grantham <algranth <@t> u.arizona.edu>
Subject: Re: [Histonet] Schiff's reagent
To: Rene J Buesa <rjbuesa <@t> yahoo.com>,Taben Hale
<taben.hale <@t> gmail.com>, histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.2.3.4.1.20071203155105.01fc65a8 <@t> algranth.inbox.email.arizona.edu>
Content-Type: text/plain; charset="iso-8859-1"; format=flowed
I'm using a Schiff's from Newcomer Supply that
has a pretty long shelf life. It can be stored at
RT and can be used several times.
We always put the "used" Schiff's into a
different bottle as to not contaminate the unused solution.
Andi Grantham
At 03:29 PM 12/3/2007, Rene J Buesa wrote:
>It has to be kept in the refrigerator, and yes, it can be used several times.
> You can test how is working by adding a few
> drops of the Schiff's reagent to NBF, it it
> reacts quickly with a magenta color, is working OK, if not, discard it.
> René J.
>
>Taben Hale <taben.hale <@t> gmail.com> wrote:
> What is the shelf-life and stability of Schiff's reagent? Can it be used
>multiple times?
>
>--
>Taben M Hale, PhD
>Postdoctoral Fellow
>Universite de Montreal
>Dept Pharmacologie
>(514)343-6111 ext. 4968
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>---------------------------------
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>you with Yahoo Mobile. Try it now.
>_______________________________________________
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>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html
------------------------------
Message: 17
Date: Mon, 3 Dec 2007 16:32:45 -0600
From: "Cheryl R. Kerry" <tkngflght <@t> yahoo.com>
Subject: RE: [Histonet] Schiff's reagent
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <00c701c835fc$6d8b3710$6901a8c0 <@t> CHERYLSLAPTOP>
Content-Type: text/plain; charset="US-ASCII"
Hello Taben-
The easiest way to determine Schiff's reactivity is to drip a little
formalin into it. Should turn BRIGHT purple. Try it fresh and remember what
it looked like for a baseline as it ages. If you're making it yourself
(great fun--don't break the flask!!)--shelf life can vary. Keep it in the
fridge and it'll last 6 mos to a year.
It CAN be used multiple times but it will be less reactive each time. Many
labs I know use it daily for two weeks at a time but you have to be careful
to verify intensity with the control tissue. More consistent results are
had by placing the slides on their backs and dropping about 2-5 ml on the
tissue for the proscribed time, then rinsing in warm running water to
develop the color and clean the slide before counterstaining.
I'm sure you'll get a variety of responses--hope this one helps.
Cheryl
Cheryl R. Kerry, HT(ASCP)
Full Staff Inc.
Staffing the AP Lab, one great tech at a time.
281.852.9457 office
281.883.7704 cell
800.756.3309 fax and alternate phone
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Taben Hale
Sent: Monday, December 03, 2007 4:26 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Schiff's reagent
What is the shelf-life and stability of Schiff's reagent? Can it be used
multiple times?
--
Taben M Hale, PhD
Postdoctoral Fellow
Universite de Montreal
Dept Pharmacologie
(514)343-6111 ext. 4968
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------------------------------
Message: 18
Date: Tue, 4 Dec 2007 01:14:17 +0000 (GMT)
From: kamal prasad <peddinti_2002us <@t> yahoo.co.in>
Subject: [Histonet] massons trichrome
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <345369.95578.qm <@t> web8703.mail.in.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Differentiate in phosphomolybdic-phosphotungstic acid solution for 15 minutes or until collagen and cytoplasm is not red. Transfer sections directly (without rinse) to aniline blue solution and stain for 5-10 minutes. Rinse briefly in distilled water and differentiate in 1% acetic acid solution for 2-5 minutes.
Also I would suggest you to use another control to confirm(Fixation and Processing).
Regards,
---------------------------------
Why delete messages? Unlimited storage is just a click away.
------------------------------
Message: 19
Date: Tue, 4 Dec 2007 14:20:56 +1100
From: "Young Kwun" <kwuny <@t> email.cs.nsw.gov.au>
Subject: [Histonet] Permanent Red stain
To: "Histonet" <histonet <@t> pathology.swmed.edu>
Message-ID: <200712041420715.SM01624 <@t> csls2816>
Content-Type: text/plain; charset="US-ASCII"
Dear Histonetters,
One of my colleagues is having a problem with Dako's Permanent Liquid Red
staining.
She told me that she was getting a heavy background around the tissues.
She is using DKDH's Untra-V-Block goat serum for blocking and the detection
system is DKSH's ONE Alkaline Phosphatase Polymer.
I use Dako's Fast Red Substrate system with Dako's labeled Polymer, AP
(anti-mouse & anti-rabbit) without such problem. I was wondering what could
cause such problems. I understand that Liquid Red is basically the same as
the Fast Red. Is that true?
Thank you in advance for your suggestion.
Young Kwun
Senior Hospital Scientist
Dept. of Anatomical Pathology
Concord Hospital
Concord NSW 2139 Australia
Tel)61-2-9767-6075
Fax)61-2-9767-8427
kwuny <@t> email.cs.nsw.gov.au
------------------------------
Message: 20
Date: Tue, 4 Dec 2007 09:09:12 +0200
From: "louise renton" <louise.renton <@t> gmail.com>
Subject: Re: [Histonet] uranium - question for microscope slide
manufacturers possibly
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<e483362e0712032309s3cb7cb43odbb7bf20e6513948 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
This is absolute free thinking here.......
could this be contamination of some sort? Perhaps the lasering process
"kicks" off some cells containing U if they are not tightly bound to the
slide and they float around to settle and upset everyones measurements
Try testing slides form a new, unopened box perferably from another distant
lab or dierctly from the supplier or another supplier altogether.
What is the background U content in Arizona? Think of the example of arsenic
in exhumed bodies. The amount found in the tissue can be rendered
meaningless if the levels in the surrounding soil around the bodies is
significant.
recalibrate the equipment with a known standard and retest
Just my 2 cents worth
On 12/4/07, Andrea Grantham <algranth <@t> u.arizona.edu> wrote:
>
> I have a question about slides containing concentrations of uranium.
>
> One of my investigators is working on a project where they are
> lasering tissue off of slides and checking it for the amount of
> uranium in the tissue. The laser actually vaporizes the tissue and it
> goes into a machine where it is tested for uranium content. They were
> getting some strange readings and tested the slides alone without
> tissue and found that the slides alone had a pretty high uranium content.
>
> So she is asking - Is there some coating to put on the slides to
> block the uranium?
>
> My question is this - is there really uranium in slides and just how
> much uranium is in a glass slide? Can this be dangerous?
>
> Are there some slides out there that do not contain uranium?
>
> I hope somebody has an answer for my weird question of the day.
>
> Thanks.
>
> Andi Grantham
> .....................................................................
> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
> : Sr. Research Specialist University of Arizona :
> : (office: AHSC 4212) P.O. Box 245044 :
> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
> : (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
> :...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
------------------------------
Message: 21
Date: Tue, 4 Dec 2007 12:04:11 -0000
From: "Kemlo Rogerson" <Kemlo.Rogerson <@t> waht.swest.nhs.uk>
Subject: RE: [Histonet] Schiff's reagent
To: "Taben Hale" <taben.hale <@t> gmail.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<86ADE4EB583CE64799A9924684A0FBBF0222F2BA <@t> wahtntex2.waht.swest.nhs.uk>
Content-Type: text/plain; charset="us-ascii"
Used to make this myself by bubbling SO2 through basic fuchsin, really
good fun, but we can't do it now.
The stock solution should be kept in the fridge and is OK when clear (if
it starts to go pink discard). We used to satin up to 5 slides in a
coplin jar which was stored in the fridge in between times. But then we
just dripped a bit onto the section as it was felt it was better to
waste small aliquots of the stain rather than risking the coplin jar
contents becoming 'weak'. Schiff's was cheapish then as we made it
ourselves but I guess it now costs a lot more made up?
Kemlo Rogerson
Pathology Manager
DD 01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
The road to success is dotted with many tempting parking places.
--Author Unknown
This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful.
Please inform me that this message has gone astray before deleting it.
Thank you for your co-operation
------------------------------
Message: 22
Date: Tue, 4 Dec 2007 04:41:52 -0800 (PST)
From: Dawn Cowie <dawn_cowie <@t> yahoo.com>
Subject: Re: [Histonet] Florida licensure question
To: Rene J Buesa <rjbuesa <@t> yahoo.com>, JMeade0710 <@t> aol.com,
bethcoxx <@t> gmail.com, histonet <@t> lists.utsouthwestern.edu
Message-ID: <668430.6557.qm <@t> web45012.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
2 companies that I have used. Florida Excel and Anderson Continuing Ed. They both sell CE materials and are recognised by state of Florida for obtaining your license. You can contact them both via internet.
Dawn
Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
Same advise, e-mail the Florida Society for Histotechnologist.
René J.
JMeade0710 <@t> aol.com wrote:
I too am a traveler, and applying for the Florida license and in the same
position that Beth is in. Any help would be appreciated.
Jerry Meade
In a message dated 12/3/2007 1:15:17 P.M. Eastern Standard Time,
bethcoxx <@t> gmail.com writes:
I work as a traveling tech, and do temporary assignments all over the
country. I want to apply for my Florida license, but I need to fulfill
that requirement for CE credits in "medical error prevention". Do any
of you Florida people have suggestions on how/where I can get that
without too much hassle?? Maybe something online? Thanks,
Beth
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Message: 23
Date: Tue, 4 Dec 2007 05:03:03 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] uranium - question for microscope slide
manufacturers possibly
To: Andrea Grantham <algranth <@t> u.arizona.edu>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <919617.96798.qm <@t> web61217.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I don't think that any coating will "block" the uranium if it is present (a physics thing).
Try to contact the manufacturer to find aout about the uranium contents of their raw (silicon) material.
René J.
Andrea Grantham <algranth <@t> u.arizona.edu> wrote:
I have a question about slides containing concentrations of uranium.
One of my investigators is working on a project where they are
lasering tissue off of slides and checking it for the amount of
uranium in the tissue. The laser actually vaporizes the tissue and it
goes into a machine where it is tested for uranium content. They were
getting some strange readings and tested the slides alone without
tissue and found that the slides alone had a pretty high uranium content.
So she is asking - Is there some coating to put on the slides to
block the uranium?
My question is this - is there really uranium in slides and just how
much uranium is in a glass slide? Can this be dangerous?
Are there some slides out there that do not contain uranium?
I hope somebody has an answer for my weird question of the day.
Thanks.
Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Be a better pen pal. Text or chat with friends inside Yahoo! Mail. See how.
------------------------------
Message: 24
Date: Tue, 4 Dec 2007 05:04:08 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Permanent Red stain
To: Young Kwun <kwuny <@t> email.cs.nsw.gov.au>, Histonet
<histonet <@t> pathology.swmed.edu>
Message-ID: <327436.88165.qm <@t> web61213.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
My advise is to contact DAKO. They are very cooperative and knowledgeable of their products.
René J.
Young Kwun <kwuny <@t> email.cs.nsw.gov.au> wrote:
Dear Histonetters,
One of my colleagues is having a problem with Dako's Permanent Liquid Red
staining.
She told me that she was getting a heavy background around the tissues.
She is using DKDH's Untra-V-Block goat serum for blocking and the detection
system is DKSH's ONE Alkaline Phosphatase Polymer.
I use Dako's Fast Red Substrate system with Dako's labeled Polymer, AP
(anti-mouse & anti-rabbit) without such problem. I was wondering what could
cause such problems. I understand that Liquid Red is basically the same as
the Fast Red. Is that true?
Thank you in advance for your suggestion.
Young Kwun
Senior Hospital Scientist
Dept. of Anatomical Pathology
Concord Hospital
Concord NSW 2139 Australia
Tel)61-2-9767-6075
Fax)61-2-9767-8427
kwuny <@t> email.cs.nsw.gov.au
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
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------------------------------
Message: 25
Date: Tue, 4 Dec 2007 07:44:29 -0600
From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
Subject: RE: [Histonet] H Pylori
To: "Mitchell, Jeannette M." <Jeannette.Mitchell <@t> vtmednet.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B3D65550856D0146B900D401EE313D4B011C1C50 <@t> dynams.dynacaremilwaukee.com>
Content-Type: text/plain; charset="iso-8859-1"
Jeannette,
We had a similar problem and solved it by switching to non-charged slides for our silver stains. It seems that the adhesive coating was taking up the stain.
Best of Luck,
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Mitchell,
Jeannette M.
Sent: Monday, December 03, 2007 3:27 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] H Pylori
Has anyone ever had dirty staining in negative gastric comtrol? I am getting some nonspecific staining in some of my gastric biopsies. The nonspecific staining I am talking about is groups of ppt., almost looks like bacteria but is way to big and is not uniform in size or clumps. I don't see it in any of the other Ipexes that we are currently performing just the gastric biopsies. Any ideas anyone??
Jeannette Mitchell, BS, HTL(ASCP), QIHC
R&D Histotechnologist
EP1-119
Fletcher Allen Health Care
Burlington, VT 05403
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------------------------------
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End of Histonet Digest, Vol 49, Issue 4
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