[Histonet] Schiff's reagent
Cheryl R. Kerry
tkngflght <@t> yahoo.com
Mon Dec 3 16:32:45 CST 2007
Hello Taben-
The easiest way to determine Schiff's reactivity is to drip a little
formalin into it. Should turn BRIGHT purple. Try it fresh and remember what
it looked like for a baseline as it ages. If you're making it yourself
(great fun--don't break the flask!!)--shelf life can vary. Keep it in the
fridge and it'll last 6 mos to a year.
It CAN be used multiple times but it will be less reactive each time. Many
labs I know use it daily for two weeks at a time but you have to be careful
to verify intensity with the control tissue. More consistent results are
had by placing the slides on their backs and dropping about 2-5 ml on the
tissue for the proscribed time, then rinsing in warm running water to
develop the color and clean the slide before counterstaining.
I'm sure you'll get a variety of responses--hope this one helps.
Cheryl
Cheryl R. Kerry, HT(ASCP)
Full Staff Inc.
Staffing the AP Lab, one great tech at a time.
281.852.9457 office
281.883.7704 cell
800.756.3309 fax and alternate phone
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Taben Hale
Sent: Monday, December 03, 2007 4:26 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Schiff's reagent
What is the shelf-life and stability of Schiff's reagent? Can it be used
multiple times?
--
Taben M Hale, PhD
Postdoctoral Fellow
Universite de Montreal
Dept Pharmacologie
(514)343-6111 ext. 4968
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