[Histonet] methanol for embedding
mesruh turkekul
turkekul <@t> gmail.com
Thu Aug 30 12:39:57 CDT 2007
Dear Colleagues
Have anyone tried methanol for dehydration for paraffin embedding?
Cheers,
Mesruh Turkekul
mskcc.org
New York, NY 10021
On 8/30/07, histonet-request <@t> lists.utsouthwestern.edu
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
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> Today's Topics:
>
> 1. RELIA Histology Job Alert (Pam Barker)
> 2. RE: [IHCRG] Re: another point on billing codes (Richard Cartun)
> 3. Re: staining mast cells and eosinophils (John Kiernan)
> 4. RE: [IHCRG] Re: another point on billing codes (Dawson, Glen)
> 5. Re: amyloid controls (Robert Richmond)
> 6. Re: CPT codes (breast) (Robert Richmond)
> 7. re: Mouse Brain IHC (Carl Hobbs)
> 8. FW: [Histonet] Periodic Acid Formalin Fixation (Denise Piontek)
> 9. Re: Re:[Histonet] IF-fading retardants (Kim Merriam)
> 10. APTS/TESPA coating, other slide coating (Emily Sours)
> 11. peloris TP (Mike Pence)
> 12. Re: APTS/TESPA coating, other slide coating (Geoff McAuliffe)
> 13. Re: APTS/TESPA coating, other slide coating (Rene J Buesa)
> 14. FW: [Histonet] APTS/TESPA coating, other slide coating
> (Ian Montgomery)
> 15. Re: peloris TP (Catherine Hill)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 29 Aug 2007 13:09:44 -0400
> From: "Pam Barker" <relia1 <@t> earthlink.net>
> Subject: [Histonet] RELIA Histology Job Alert
> To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <E1IQR32-00007q-CR <@t> elasmtp-junco.atl.sa.earthlink.net>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi Histonetters,
> I have several new positions that I am excited to tell you about. Here
> is the information:
> I am currently working with a client in the Los Angeles area that is in
> need of 2 histo techs with IHC experience. I also have a client in the
> Santa Barbara area in need of a histology manager and I have a client in
> Northern Virginia in need of a histotech These are permanent full time
> dayshift positions. The clients offers a great environment, a great
> crew to work with and excellent salary, benefits and relocation
> assistance.
>
> Experienced Techs and New Grads are welcome to apply.
>
> My question is do you know of anyone who might be interested in these
> positions?
>
> If you think you or someone you know might be interested please contact
> me.
>
> I can be reached at 866-607-3542 or relia1 <@t> earthlink.net Thanks-Pam
>
>
>
> Thank You!
>
>
>
> Pam Barker
> President
> RELIA
> Specialists in Allied Healthcare Recruiting
> 5703 Red Bug Lake Road #330
> Winter Springs, FL 32708-4969
> Phone: (407)657-2027
> Cell: (407)353-5070
> FAX: (407)678-2788
> E-mail: relia1 <@t> earthlink.net
>
>
> ------------------------------
>
> Message: 2
> Date: Wed, 29 Aug 2007 13:22:40 -0400
> From: "Richard Cartun" <Rcartun <@t> harthosp.org>
> Subject: RE: [Histonet] [IHCRG] Re: another point on billing codes
> To: <rorr <@t> enh.org>,<pruegg <@t> ihctech.net>,
> <histonet <@t> lists.utsouthwestern.edu>, "Joyce Weems" <JWEEMS <@t> sjha.org>
> Cc: "IHCRG Resource Group \(E-mail\)" <ihcrg <@t> googlegroups.com>
> Message-ID: <46D573200200007700007C83 <@t> gwmail1.harthosp.org>
> Content-Type: text/plain; charset=US-ASCII
>
> Yes, you're correct. However, in my opinion, the increased RVU for the technical component of 88361 in not justified.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
> >>> "Weems, Joyce" <JWEEMS <@t> sjha.org> 08/29/07 11:59 AM >>>
> Except for quantitative analysis, the instructions in the AMA CPT codebook are NOT to report 88342 with 88360 and 88361 unless each procedure is for a different antibody. 88360 - manual, 88361 - computer assisted technology.
>
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital
> 5665 Peachtree Dunwoody Rd NE
> Atlanta, GA 30342
> 404-851-7376 - Phone
> 404-851-7831 - Fax
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Richard
> Cartun
> Sent: Wednesday, August 29, 2007 11:35 AM
> To: rorr <@t> enh.org; pruegg <@t> ihctech.net; histonet <@t> lists.utsouthwestern.edu
> Cc: 'IHCRG Resource Group (E-mail)'
> Subject: [Histonet] [IHCRG] Re: another point on billing codes
>
>
> I also believe that 88360 or 88361 should be used for professional interpretation (and billing) only; the technical should always be 88342 since you are not doing anything different to the slide whether you look at it under the microscope or you use image analysis. Only my opinion .......
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
> >>> "Patsy Ruegg" <pruegg <@t> ihctech.net> 08/29/07 11:05 AM >>>
>
> All,
> I find this thread interesting and was wondering if I have everyone's
> permission to use this as one of the questions for the NSH IHC Forum?
> Patsy
>
> -----Original Message-----
> From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf Of
> Richard Cartun
> Sent: Wednesday, August 29, 2007 6:49 AM
> To: rorr <@t> enh.org; histonet <@t> lists.utsouthwestern.edu
> Cc: IHCRG Resource Group (E-mail)
> Subject: [IHCRG] Re: another point on billing codes
>
>
> Interesting point. We use semiquantitative scoring for ER, PR, and HER2
> performed on primary breast CAs. Therefore, we use 88360x3 for these
> markers whether they are positive or negative.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
> >>> "Orr, Rebecca" <ROrr <@t> enh.org> 08/29/07 7:24 AM >>>
>
> Helayne,
> I'm interested in everyone's input on this thread.
> Charging for Breast cases seems to be as unclear as the processing
> guidelines.
> We are now in the process of figuring out charges if the ER PR Her2
> results are negative.
>
> ER PR Her2 are quantitative (2+, 3+) or semi quantitative (weakly or
> strongly positive, etc)
> Assuming these markers are ordered on a breast cancer (not a benign
> breast), even a negative result is quantitative and contributes to the
> outcome of the therapy., isn't this right?
> Please steer me in the right direction if this is an incorrect point.
>
> So we are being told by our billing folks that we must change the code
> on the negative resulted ER PR her2 to a lesser charge.
> I can understand if CPT may think doctors are charging on unnecessary
> IHC tests, but they are focusing on the wrong tests.
> (in my opinion).
> A negative or 0 result on these particular markers should NOT be
> synonymous with "
>
> Quite perplexing and frustrating.
>
>
> ---Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu
> > [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Parker,
> > Helayne
> > Sent: Tuesday, August 28, 2007 4:25 PM
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: [Histonet] CPT codes
> >
> >
> > Hi all,
> > Does anyone know the correct charges to charge a breast lump that
> must
> > be inked. Someone told us we could only charge a -307 if it has
> cancer
> > in the micro margins. As much gross work is done either way (cancer
> or
> > not) so what is the real truth ? We have given most lumps 305 and I
> am
> > thinking we are undercharging.
> >
> > Thanks,
> > Helayne Parker, HT (ASCP)
> > Histology Section Head
> > Skaggs Community Health Center
> > Branson, Missouri
> Becky Orr CLA,HT(ASCP)QIHC
> Anatomic Pathology
> Evanston Northwestern Healthcare
> 847-570-2771
>
>
>
>
>
>
>
>
> --~--~---------~--~----~------------~-------~--~----~
> You received this message because you are subscribed to the Google Groups "ihcrg" group.
> To post to this group, send email to ihcrg <@t> googlegroups.com
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>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc.
>
>
>
> ------------------------------
>
> Message: 3
> Date: Wed, 29 Aug 2007 13:51:16 -0400
> From: John Kiernan <jkiernan <@t> uwo.ca>
> Subject: Re: [Histonet] staining mast cells and eosinophils
> To: Moran Elishmereni <moran.elish <@t> gmail.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <de06a78343d2b.46d579d4 <@t> uwo.ca>
> Content-Type: text/plain; charset=us-ascii
>
> Yes, it's easy to stain both cell-types. First stain with eosin at a high pH (such as 8) to obtain selective coloration of eosinophils. (Paneth cell granules will also stain if your tissue is intestine.) Wash in slightly acidified water, then stain with dilute toluidine blue at a low pH (less than 2 to be selective for mast cells, cartilage matrix and some types of mucus). The staining is metachromatic - purple to red, rather than blue, but not likely to be confused with the eosin colour. Rinse in slightly acidified water, dehydrate in 3 changes of 100% alcohol, clear in xylene and apply coverslip.
> Slightly acidified water = approx 0.5% acetic acid. Check with a microscope at both the washing stages to make sure the eosinophils and mast cells are adequately stained. As described above, nuclei should be unstained. If you want blue nuclei, use the toluidine blue at pH 3 to 4, being careful to avoid over-staining.
>
> John Kiernan
> Anatomy, UWO
> London, Canada.
> -----
> ----- Original Message -----
> From: Moran Elishmereni <moran.elish <@t> gmail.com>
> Date: Tuesday, August 28, 2007 5:36
> Subject: [Histonet] staining mast cells and eosinophils
> To: histonet <@t> lists.utsouthwestern.edu
>
> > Hello,
> >
> > I am very new at histology and immunohistochemistry, so please
> > excuse the
> > simplicity of my questions. I wish to stain parrafin-embedded slides
> > (murine/human skin and lung) for mast cells and eosinophils.
> > That is- I want
> > to be able to detect both cells on one slide. Is there anyway to
> > stain with
> > toluidine blue and also counterstain with another dye for eosinophils?
> > Alternatively- can i stain for toluidine blue to get mast cells,
> > and then do
> > (on the same slide) immunohistochemistry using an anti-MBP
> > antibody to
> > detect eosinophils? I guess its more of a general question- can
> > one use a
> > simple dye AND immunohistochemistry (with antibodies) on the
> > same slide, or
> > is there interference? I would be very glad to receive advice
> > and protocols.
> >
> > Many thanks,
> > Moran Elishmereni
> >
> > Department of Pharmacology
> > School of Pharmacy, Faculty of Medicine
> > The Hebrew University of Jerusalem
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
> ------------------------------
>
> Message: 4
> Date: Wed, 29 Aug 2007 13:16:33 -0500
> From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
> Subject: RE: [Histonet] [IHCRG] Re: another point on billing codes
> To: <histonet <@t> lists.utsouthwestern.edu>
> Cc: "IHCRG Resource Group \(E-mail\)" <ihcrg <@t> googlegroups.com>
> Message-ID:
> <B3D65550856D0146B900D401EE313D4BE7FBFF <@t> dynams.dynacaremilwaukee.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> If you use an FDA approved kit for the staining which not only costs much more than a routine 88342, but also requires more steps that may not fit into an IHC lab's routine protocol (since you cannot alter any step in the kit) calling for special handling and more labor, I can see justification in increased RVU for the technical component. I currently use FDA approved kits for HercepTest & EGFR staining and I can say, without question, that the technical cost, in terms of both labor and reagents, is more than when I did these two IHC's before bringing in the kits.
>
> My Opinion,
>
> Glen Dawson
> IHC Manager
> Milwaukee, WI
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Richard
> Cartun
> Sent: Wednesday, August 29, 2007 12:23 PM
> To: rorr <@t> enh.org; pruegg <@t> ihctech.net; histonet <@t> lists.utsouthwestern.edu;
> Joyce Weems
> Cc: IHCRG Resource Group (E-mail)
> Subject: RE: [Histonet] [IHCRG] Re: another point on billing codes
>
>
> Yes, you're correct. However, in my opinion, the increased RVU for the technical component of 88361 in not justified.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
> >>> "Weems, Joyce" <JWEEMS <@t> sjha.org> 08/29/07 11:59 AM >>>
> Except for quantitative analysis, the instructions in the AMA CPT codebook are NOT to report 88342 with 88360 and 88361 unless each procedure is for a different antibody. 88360 - manual, 88361 - computer assisted technology.
>
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital
> 5665 Peachtree Dunwoody Rd NE
> Atlanta, GA 30342
> 404-851-7376 - Phone
> 404-851-7831 - Fax
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Richard
> Cartun
> Sent: Wednesday, August 29, 2007 11:35 AM
> To: rorr <@t> enh.org; pruegg <@t> ihctech.net; histonet <@t> lists.utsouthwestern.edu
> Cc: 'IHCRG Resource Group (E-mail)'
> Subject: [Histonet] [IHCRG] Re: another point on billing codes
>
>
> I also believe that 88360 or 88361 should be used for professional interpretation (and billing) only; the technical should always be 88342 since you are not doing anything different to the slide whether you look at it under the microscope or you use image analysis. Only my opinion .......
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
> >>> "Patsy Ruegg" <pruegg <@t> ihctech.net> 08/29/07 11:05 AM >>>
>
> All,
> I find this thread interesting and was wondering if I have everyone's
> permission to use this as one of the questions for the NSH IHC Forum?
> Patsy
>
> -----Original Message-----
> From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf Of
> Richard Cartun
> Sent: Wednesday, August 29, 2007 6:49 AM
> To: rorr <@t> enh.org; histonet <@t> lists.utsouthwestern.edu
> Cc: IHCRG Resource Group (E-mail)
> Subject: [IHCRG] Re: another point on billing codes
>
>
> Interesting point. We use semiquantitative scoring for ER, PR, and HER2
> performed on primary breast CAs. Therefore, we use 88360x3 for these
> markers whether they are positive or negative.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
> >>> "Orr, Rebecca" <ROrr <@t> enh.org> 08/29/07 7:24 AM >>>
>
> Helayne,
> I'm interested in everyone's input on this thread.
> Charging for Breast cases seems to be as unclear as the processing
> guidelines.
> We are now in the process of figuring out charges if the ER PR Her2
> results are negative.
>
> ER PR Her2 are quantitative (2+, 3+) or semi quantitative (weakly or
> strongly positive, etc)
> Assuming these markers are ordered on a breast cancer (not a benign
> breast), even a negative result is quantitative and contributes to the
> outcome of the therapy., isn't this right?
> Please steer me in the right direction if this is an incorrect point.
>
> So we are being told by our billing folks that we must change the code
> on the negative resulted ER PR her2 to a lesser charge.
> I can understand if CPT may think doctors are charging on unnecessary
> IHC tests, but they are focusing on the wrong tests.
> (in my opinion).
> A negative or 0 result on these particular markers should NOT be
> synonymous with "
>
> Quite perplexing and frustrating.
>
>
> ---Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu
> > [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Parker,
> > Helayne
> > Sent: Tuesday, August 28, 2007 4:25 PM
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: [Histonet] CPT codes
> >
> >
> > Hi all,
> > Does anyone know the correct charges to charge a breast lump that
> must
> > be inked. Someone told us we could only charge a -307 if it has
> cancer
> > in the micro margins. As much gross work is done either way (cancer
> or
> > not) so what is the real truth ? We have given most lumps 305 and I
> am
> > thinking we are undercharging.
> >
> > Thanks,
> > Helayne Parker, HT (ASCP)
> > Histology Section Head
> > Skaggs Community Health Center
> > Branson, Missouri
> Becky Orr CLA,HT(ASCP)QIHC
> Anatomic Pathology
> Evanston Northwestern Healthcare
> 847-570-2771
>
>
>
>
>
>
>
>
> --~--~---------~--~----~------------~-------~--~----~
> You received this message because you are subscribed to the Google Groups "ihcrg" group.
> To post to this group, send email to ihcrg <@t> googlegroups.com
> To unsubscribe from this group, send email to ihcrg-unsubscribe <@t> googlegroups.com
> For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en
> -~----------~----~----~----~------~----~------~--~---
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 5
> Date: Wed, 29 Aug 2007 14:25:26 -0400
> From: "Robert Richmond" <RSRICHMOND <@t> aol.com>
> Subject: [Histonet] Re: amyloid controls
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <abea52a60708291125u653cf0edrbc422012c94382a8 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Steve Coakley asks about amyloid controls. They're notoriously
> difficult to get, and unstained sections don't keep well.
>
> What I want to know is - there are a number of methods for producing
> amyloidosis in experimental animals (casein injection in mice was a
> favorite) in the older literature. Is this animal amyloid suitable for
> use as an amyloid control? If it is, why isn't it available?
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
>
>
> ------------------------------
>
> Message: 6
> Date: Wed, 29 Aug 2007 15:06:08 -0400
> From: "Robert Richmond" <RSRICHMOND <@t> aol.com>
> Subject: [Histonet] Re: CPT codes (breast)
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <abea52a60708291206t167371cdtc25812346df78a52 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Here's chapter and verse from the College of American Pathologists
> (CAP) Web site, originally published in the June 2003 CAP TODAY. I
> confess I don't find it exactly clear!
>
> Q: Is an oriented needle-localization lumpectomy specimen, which turns
> out to be benign, coded as 88305 or 88307?
>
> A: The final diagnosis is not the sole determining factor in assigning
> the appropriate CPT code for breast excisions. If the clinical
> assessment and mammography findings determine the need to assess
> adequacy of the excision (with microscopic evaluation of the margins),
> and the material is prepared and evaluated, then it should be coded
> accordingly. In this scenario the appropriate code is 88307 Breast,
> Excision of Lesion, Requiring Microscopic Evaluation of Surgical
> Margins.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
>
>
> ------------------------------
>
> Message: 7
> Date: Wed, 29 Aug 2007 20:37:46 +0100
> From: "Carl Hobbs" <carl.hobbs <@t> kcl.ac.uk>
> Subject: [Histonet] re: Mouse Brain IHC
> To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000901c7ea74$13ee51c0$4101a8c0 <@t> carlba65530bda>
> Content-Type: text/plain; format=flowed; charset="iso-8859-1";
> reply-type=original
>
> I have used Abcam's TGF beta receptor 1 on pwax sections: pic here....
> http://www.immunoportal.com/modules.php?set_albumName=album01&op=modload&name=Gallery&file=index&include=view_album.php
> Carl
>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Wed, 29 Aug 2007 16:47:21 -0400
> From: Denise Piontek <dbpiontek <@t> hotmail.com>
> Subject: FW: [Histonet] Periodic Acid Formalin Fixation
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BAY134-W28BF028B4E5965AE10DD90ABCC0 <@t> phx.gbl>
> Content-Type: text/plain; charset="Windows-1252"
>
>
> > > > Dear Histonetters:> I am performing Periodic Acid Formalin fixation on liver and noticing sinusoid shrinkage, as well as some edge effect? This is in comparison to a matched tissue control in 10% NBF. My recipe requires fixation at 4C for 48 hours via 1 gram of periodic acid in 100 mls 10% NBF. Anyone else using this fixation method? Any suggestions or advice is greatly appreciated,> > Denise Bland-Piontek, HTL(ASCP)CTBS(AATB)> NIBRI
> _________________________________________________________________
> Messenger Café — open for fun 24/7. Hot games, cool activities served daily. Visit now.
> http://cafemessenger.com?ocid=TXT_TAGLM_AugWLtagline
>
> ------------------------------
>
> Message: 9
> Date: Thu, 30 Aug 2007 06:08:25 -0700 (PDT)
> From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
> Subject: Re: Re:[Histonet] IF-fading retardants
> To: John Kiernan <jkiernan <@t> uwo.ca>, Histonet
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <976643.40560.qm <@t> web50311.mail.re2.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> John,
>
> Are you saying that IF stained slides can go through the normal dehydration/clearing process and be mounted in a xylene-based mounting medium?
>
> Kim
>
> Kim Merriam, MA, HT(ASCP)
> Cambridge, MA
>
>
>
> ----- Original Message ----
> From: John Kiernan <jkiernan <@t> uwo.ca>
> To: AGrobe2555 <@t> aol.com
> Cc: histonet <@t> lists.utsouthwestern.edu
> Sent: Wednesday, August 29, 2007 1:31:52 AM
> Subject: Re: Re:[Histonet] IF-fading retardants
>
>
> Why do you "suspect you would kill your signal" by dehydrating, clearing and mounting a slide carrying a fluorescently labelled antibody?
>
> Alcohol coagulates proteins on-the-spot, complete with any covalently bound fluorescent tags. This was established for lectin histochemistry 30 years ago, and has been well documented for fluorescently labelled antibodies in more recent years. The intensity of fluorescence emission may be less in DPX than in buffered glycerol, but who has done comparisons?
>
> A fairly recent study strongly favours alcohol dehydration, clearing and mounting in a non-flourescent recinous medium. for permanent immunofluorescence slides.
>
> John Kiernan
> Anatomy, UWO
> London, Canada
> ---
> ----- Original Message -----
> From: AGrobe2555 <@t> aol.com
> Date: Monday, August 27, 2007 14:31
> Subject: Re:[Histonet] IF-fading retardants
> To: histonet <@t> lists.utsouthwestern.edu
>
> > Carl,
> > I used to use "FluorSave" mounting medium from Calbiochem (Cat
> > # 345789).
> > This mounting medium "hardens" so the coverslip doesn't
> > move and you don't
> > have to seal around the edges with nail polish. It also
> > kept the signal from
> > fading. I still stored the slides horizontally at 4C
> > or -20C. I wouldn't
> > suggest try in to dehydrate through xylene and
> > coverslipping, as I suspect you
> > would kill your signal.
> > Albert
> >
> >
> > Albert C. Grobe, PhD
> > International Heart Institute of Montana Foundation
> > Tissue Engineering Lab, Saint Patrick Hospital
> >
> >
> >
> >
> >
> > ************************************** Get a sneak peek of the
> > all-new AOL at
> > http://discover.aol.com/memed/aolcom30tour
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ____________________________________________________________________________________
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> to amazing places on Yahoo! Travel.
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>
> ------------------------------
>
> Message: 10
> Date: Thu, 30 Aug 2007 10:16:39 -0400
> From: "Emily Sours" <talulahgosh <@t> gmail.com>
> Subject: [Histonet] APTS/TESPA coating, other slide coating
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <b39794b0708300716l117007baj902cb0693253da18 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> On this note, has anyone noticed that egg albumin-coated slides are better
> than TESPA (3-aminopropyltriethoxysilane)
> -coated slides for paraffin sectioning? We're using them for H&E and IHC. I
> assume that egg-albumin would have more background in IHC, but I haven't
> tried it yet.
>
> Emily
> --
> Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and
> somebody actually proposed calling German measles, 'Liberty
> measles?'...Remember when the hick legislators in certain states, in
> obedience to William Jennings Bryan, who learned his biology from his pious
> old grandma, set up shop as scientific experts and made the whole world
> laugh itself sick by forbidding the teaching of evolution?
> --Sinclair Lewis, It Can't Happen Here, 1935
>
>
> ------------------------------
>
> Message: 11
> Date: Thu, 30 Aug 2007 10:04:23 -0500
> From: "Mike Pence" <mpence <@t> grhs.net>
> Subject: [Histonet] peloris TP
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C6F0 <@t> IS-E2K3.grhs.net>
> Content-Type: text/plain; charset="us-ascii"
>
> Peloris Users:
>
> I have searched the archives and read the past about users of the
> Peloris. We are in the market for a new TP and are looking at the
> Peloris as one of our chooses. I would like to hear from users that
> have had their TP for some time and the Pros-Cons of the unit. The
> vendors only give you references of those they want you to call. I want
> to hear from those they don't want you to call. I would ask that you
> please reply off-line to protect you from being "flamed".
>
> Mike Pence
> AP Supervisor
> Great River Medical Center
> mpence <@t> grhs.net
>
>
>
> ------------------------------
>
> Message: 12
> Date: Thu, 30 Aug 2007 11:09:42 -0400
> From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
> Subject: Re: [Histonet] APTS/TESPA coating, other slide coating
> To: Emily Sours <talulahgosh <@t> gmail.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <46D6DDB6.6090903 <@t> umdnj.edu>
> Content-Type: text/plain; format=flowed; charset=ISO-8859-1
>
> I think 'silane' slides are better for paraffin sections. The harsher
> the treatment, the better 'silane' is.
>
> Geoff
>
> Emily Sours wrote:
> > On this note, has anyone noticed that egg albumin-coated slides are better
> > than TESPA (3-aminopropyltriethoxysilane)
> > -coated slides for paraffin sectioning? We're using them for H&E and IHC. I
> > assume that egg-albumin would have more background in IHC, but I haven't
> > tried it yet.
> >
> > Emily
> >
>
>
> --
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583
> mcauliff <@t> umdnj.edu
> **********************************************
>
>
>
>
>
> ------------------------------
>
> Message: 13
> Date: Thu, 30 Aug 2007 08:54:13 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] APTS/TESPA coating, other slide coating
> To: Emily Sours <talulahgosh <@t> gmail.com>,
> histonet <@t> lists.utsouthwestern.edu
> Message-ID: <314106.50588.qm <@t> web61224.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Even if you dilute the albumen after filtering it (during preparation), you will have an increase in background noise.
> René J
>
> Emily Sours <talulahgosh <@t> gmail.com> wrote:
> On this note, has anyone noticed that egg albumin-coated slides are better
> than TESPA (3-aminopropyltriethoxysilane)
> -coated slides for paraffin sectioning? We're using them for H&E and IHC. I
> assume that egg-albumin would have more background in IHC, but I haven't
> tried it yet.
>
> Emily
> --
> Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and
> somebody actually proposed calling German measles, 'Liberty
> measles?'...Remember when the hick legislators in certain states, in
> obedience to William Jennings Bryan, who learned his biology from his pious
> old grandma, set up shop as scientific experts and made the whole world
> laugh itself sick by forbidding the teaching of evolution?
> --Sinclair Lewis, It Can't Happen Here, 1935
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV.
>
> ------------------------------
>
> Message: 14
> Date: Thu, 30 Aug 2007 17:12:29 +0100
> From: "Ian Montgomery" <ian.montgomery <@t> bio.gla.ac.uk>
> Subject: FW: [Histonet] APTS/TESPA coating, other slide coating
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <003201c7eb20$907a1830$6424d182 <@t> IBLS.GLA.AC.UK>
> Content-Type: text/plain; charset="us-ascii"
>
> Geoff,
> As someone who routinely uses poly-l-lysine, I'm interested in your
> comment that silane is better for paraffin sections, why? I've also had a
> quick browse at the techniques for coating and although almost identical,
> the times in silane range from 10 seconds, Gibbs,L. Histonet Sept 06, 2001
> to 5 minutes, Mutter 1988. Any thoughts on time?
> Ian.
>
>
>
> I think 'silane' slides are better for paraffin sections. The harsher
> the treatment, the better 'silane' is.
>
> Geoff
>
> Emily Sours wrote:
> > On this note, has anyone noticed that egg albumin-coated slides are better
> > than TESPA (3-aminopropyltriethoxysilane)
> > -coated slides for paraffin sectioning? We're using them for H&E and IHC.
> I
> > assume that egg-albumin would have more background in IHC, but I haven't
> > tried it yet.
> >
> > Emily
> >
>
>
> --
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583
> mcauliff <@t> umdnj.edu
> **********************************************
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> Dr. Ian Montgomery,
> Histotechnology,
> I.B.L.S. Support Unit,
> Thomson Building,
> University of Glasgow,
> G12 8QQ.
>
>
>
>
> ------------------------------
>
> Message: 15
> Date: Thu, 30 Aug 2007 12:12:59 -0400
> From: "Catherine Hill" <chill <@t> amh.org>
> Subject: Re: [Histonet] peloris TP
> To: mpence <@t> grhs.net, histonet <@t> lists.utsouthwestern.edu
> Message-ID: <s6d6b45d.027 <@t> hosp.amh.org>
> Content-Type: TEXT/plain; charset="US-ASCII"
>
> Mike,
> If you would share the information you receive, or if responders would copy to me, Iwould appreciate it. We are considering purchasing a Peloris.
>
> Catherine Hill, CT(ASCP) (IAC)
> Anatomic Pathology Manager
> Abington Memorial Hospital
> 215-481-4393
> chill <@t> amh.org
>
> >>> "Mike Pence" <mpence <@t> grhs.net> 8/30/2007 11:04 AM >>>
> Peloris Users:
>
> I have searched the archives and read the past about users of the
> Peloris. We are in the market for a new TP and are looking at the
> Peloris as one of our chooses. I would like to hear from users that
> have had their TP for some time and the Pros-Cons of the unit. The
> vendors only give you references of those they want you to call. I want
> to hear from those they don't want you to call. I would ask that you
> please reply off-line to protect you from being "flamed".
>
> Mike Pence
> AP Supervisor
> Great River Medical Center
> mpence <@t> grhs.net
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
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> End of Histonet Digest, Vol 45, Issue 45
> ****************************************
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