[Histonet] staining mast cells and eosinophils

[Moran Elishmereni]

Thanks. Can I stain the same section for toluidine blue AND eosin?

Moran


On 8/28/07, Tarango, Mark <mtarango <@t> nvcancer.org> wrote:
>
>
> Try doing the IHC for eosinophils first and do a toluene blue for mast
> cells as a counter stain.
>
>
> Mark Adam Tarango HT(ASCP)
>
> Histology & IHC Supervisor
>
> Nevada Cancer Institute
>
> One Breakthrough Way
>
> Las Vegas, NV  89135
>
> Direct Line (702) 822-5112
>
> Mobile (702) 759-9229
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> Fax (702) 939-7663
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>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Moran
> Elishmereni
> Sent: Tuesday, August 28, 2007 2:34 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] staining mast cells and eosinophils
>
> Hello,
>
> I am very new at histology and immunohistochemistry, so please excuse
> the
> simplicity of my questions. I wish to stain parrafin-embedded slides
> (murine/human skin and lung) for mast cells and eosinophils. That is- I
> want
> to be able to detect both cells on one slide. Is there anyway to stain
> with
> toluidine blue and also counterstain with another dye for eosinophils?
> Alternatively- can i stain for toluidine blue to get mast cells, and
> then do
> (on the same slide) immunohistochemistry using an anti-MBP antibody to
> detect eosinophils? I guess its more of a general question- can one use
> a
> simple dye AND immunohistochemistry (with antibodies) on the same slide,
> or
> is there interference? I would be very glad to receive advice and
> protocols.
>
> Many thanks,
> Moran Elishmereni
>
> Department of Pharmacology
> School of Pharmacy, Faculty of Medicine
> The Hebrew University of Jerusalem
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> "EMF <nvcancer.org>" made the following annotations.
>
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-- 
Moran Elishmereni

Department of Pharmacology
School of Pharmacy, Faculty of Medicine
The Hebrew University of Jerusalem
POB 12065
Jerusalem 91120, ISRAEL
Tel: 972-2-675-8746
Fax: 972-2-675-8144
Email: moran.elish <@t> gmail.com