[Histonet] RE: ISH

[Langxing Pan]

Hi Naira,

Many years ago, I happened to leave a few unfixed frozen sections at room
temperature over a weekend. I then fixed and hybridized these sections
together with some freshly cut/fixed frozen sections with a riboprobe to Ig
kappa light chain mRNA. I did not see much difference in signals among the
sections, but noted that the morphology of the dried sections was much
better than that of the fresh ones. Since then we dry routinely our frozen
sections and cell cytospins for at least one hour before fixation for IHC
and ISH. 

Langxing


Langxing Pan, M.D., Ph.D.
Pantomics, Inc.
457 Mariposa Street
San Francisco
CA 94107, U.S.A.
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www.pantomics.com