[Histonet] Dendritic Cells/Mast Cells/NK Cells on FFPE mouse

[Tarango, Mark]

I forgot to mention CD68 dendritic cells.  CD68 (KP-1 or ham-56) will
stain macrophages and monocytes too.  You kind of have to know what
you're looking at.  Dendritic cells can be from more than just one
lineage, and CD68 might only pick up the myeloid-related ones.  There is
an antibody against follicular dendritic cells from cell marque too.  I
can't really think of just one marker for dendritic cells.  You'd be
better off using more than 1 marker and calling a dendritic cell a
dendritic cell based of a panel of antibodies.  Maybe immunofloresence
would be best here.

I personally like CD68 (ham56 clone) because you can kind of see the
long projections that dendritic cells have with ham-56.  KP-1 looks like
spray paint and ham-56 looks like oil painting or something.  It's kind
of funny since they're both CD68. 


Mark Adam Tarango HT(ASCP)

Histology & IHC Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV  89135

Direct Line (702) 822-5112

Mobile (702) 759-9229

Fax (702) 939-7663

  

-----Original Message-----
From: Tarango, Mark 
Sent: Monday, August 27, 2007 2:39 PM
To: 'Larry Woody'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Dendritic Cells/Mast Cells/NK Cells on FFPE
mouse

You could do a toluene blue for mast cells.  Maybe you could stain for
IgE too, but I really don't know for sure.  If you want an antibody, I'd
say C-kit (CD117) is the best...but you've already done that.  You could
use CD56 for natural killers.


Mark Adam Tarango HT(ASCP)

Histology & IHC Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV  89135

Direct Line (702) 822-5112

Mobile (702) 759-9229

Fax (702) 939-7663

  


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Larry
Woody
Sent: Monday, August 27, 2007 12:47 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Dendritic Cells/Mast Cells/NK Cells on FFPE mouse

Looking for antibodies for these targets on FFPE mouse. We've tried
CD117,Tryptase and Chymase already. Thanks
       
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"EMF <nvcancer.org>" made the following annotations.
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