[Histonet] IF staining: can I mount pwax sections in DPX?
Carl Hobbs
carl.hobbs <@t> kcl.ac.uk
Sat Aug 25 14:11:10 CDT 2007
Hi.
I usually carry out single/double IF-staining on pwax sections and coverslip
sections in an aq. anti-fade mountant.
This works well.
However, can these sections be mounted in a solvent-based mountant and not
only retain IF but also be able to retard fading? ( eg: DPX, after
dehydrating in alcohol, clearing and mounting in xylene)
Carl
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