[Histonet] platelet EM

[Philip Oshel]

TEM or SEM?
Fully spread platelets can be done as whole mounts by either method. 
SEM requires low kV accelerating voltages, less than 3 kV, 1.5 or 
lower is better (platelets are basically invisible in the SEM at 10 
kV and above, the beam goes right through them).
Ralph Albrecht et al in the 80s and 90s published several articles on 
platelets using SEM and TEM. I'd look up his papers/book chapters.

Phil

>Does anyone have a procedure for preparing platelets for EM examination?
>

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Thanks
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Ronnie
>

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Ronnie Houston, MS, HT(ASCP)QIHC
>
Anatomic Pathology Manager
>
Columbus Children's Hospital
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700 Children's Drive
>
Columbus, OH 43205
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(614) 722 5465
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houstonr <@t> chi.osu.edu
-- 
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859