[Histonet] antibody diluent

[Rene J Buesa]

If used above the expiration date it will cause what you describe. You will have to buy a new one of prepare your own with the following recipe:
  1- bovine serum--- 1 mL
  2- PBS buffer --- 99 mL
  3- Tween 20 ---- 0,05 mL
  4- sodium azide --- 100 mg
  Store at 4ºC
  René J.

Kimberly Tuttle <ktuttle <@t> umm.edu> wrote:
  Can antibody diluent go bad, and cause non-specific staining? I have no idea how old it is and theres no expiration date.





This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail.


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


       
---------------------------------
Boardwalk for $500? In 2007? Ha! 
Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games.