[Histonet] antibody diluent

Rene J Buesa rjbuesa <@t> yahoo.com
Wed Aug 22 11:48:27 CDT 2007

If used above the expiration date it will cause what you describe. You will have to buy a new one of prepare your own with the following recipe:
  1- bovine serum--- 1 mL
  2- PBS buffer --- 99 mL
  3- Tween 20 ---- 0,05 mL
  4- sodium azide --- 100 mg
  Store at 4ºC
  René J.

Kimberly Tuttle <ktuttle <@t> umm.edu> wrote:
  Can antibody diluent go bad, and cause non-specific staining? I have no idea how old it is and theres no expiration date.

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