[Histonet] IF staining of mast cells and fibroblasts

[Kim Merriam]

Thanks to everyone who responded.  Once again, the histonet proves to be an invaluable resource!
 
Kim


Kim Merriam, MA, HT(ASCP)
Cambridge, MA



----- Original Message ----
From: Bernice Frederick <b-frederick <@t> northwestern.edu>
To: Liz Chlipala <liz <@t> premierlab.com>; Kim Merriam <kmerriam2003 <@t> yahoo.com>; Histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, August 20, 2007 1:59:01 PM
Subject: RE: [Histonet] IF staining of mast cells and fibroblasts


Mast cell tryptase works fine on human 1:8000.

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz
Chlipala
Sent: Monday, August 20, 2007 11:20 AM
To: Kim Merriam; Histonet
Subject: RE: [Histonet] IF staining of mast cells and fibroblasts

Kim

Mast cell tryptase works in mice also, so I suspect it might work in
other species.  For fibroblasts there is a marker that works in rat its
mouse anti rat prolyl-4-hydroxylase beta (fibroblast marker) antibody
6-9H6 (AF5110-1) from Acris. 

Liz 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim
Merriam
Sent: Monday, August 20, 2007 9:46 AM
To: Histonet
Subject: [Histonet] IF staining of mast cells and fibroblasts

Hello all,

I was wondering if anyone knew of specific markers for mast cells and
fibroblasts.  I need markers for IF and IHC (not special stains) that
will stain only mast cells and only fibroblasts.  We have a few markers
in mind, but we are not sure if they are specific only to those types of
cells and will not cross-react with some other cell type.

Thanks in advance,

Kim

Kim Merriam, MA, HT(ASCP)
Cambridge, MA


       
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