[Histonet] IF staining of mast cells and fibroblasts

[Liz Chlipala]

Kim

Mast cell tryptase works in mice also, so I suspect it might work in =
other species.  For fibroblasts there is a marker that works in rat its =
mouse anti rat prolyl-4-hydroxylase beta (fibroblast marker) antibody =
6-9H6 (AF5110-1) from Acris.=20

Liz=20

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
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Ship to Address:
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Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu =
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim =
Merriam
Sent: Monday, August 20, 2007 9:46 AM
To: Histonet
Subject: [Histonet] IF staining of mast cells and fibroblasts

Hello all,

I was wondering if anyone knew of specific markers for mast cells and =
fibroblasts.  I need markers for IF and IHC (not special stains) that =
will stain only mast cells and only fibroblasts.  We have a few markers =
in mind, but we are not sure if they are specific only to those types of =
cells and will not cross-react with some other cell type.

Thanks in advance,

Kim
=20
Kim Merriam, MA, HT(ASCP)
Cambridge, MA


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