[Histonet] alcohol fixation image (2nd try)

[kemlo]

Few words?

Formalin takes longer to fix but is a 'soft' fixative; alcohol is a faster
fixative but hardens and shrinks tissue if it's left in it too long.
Formalin is an additive coagulant fixative (forms bonds between proteins)
whilst alcohol is a non-additive coagulant fixative (does something with the
tertiary structure of proteins). Tissue needs formalin, cells are OK with
alcohol; Cytology fixatives tend to be alcoholic whilst Histology Fixatives
aren't. A wide generalisation I know but words are sacred <g>.



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: 18 August 2007 18:14
To: histonet <@t> lists.utsouthwestern.edu
Subject: AW: [Histonet] alcohol fixation image (2nd try)

Uups, should be "formalin and alcohol-fixed".

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Gudrun
Lang
Gesendet: Samstag, 18. August 2007 19:05
An: 'Geoff McAuliffe'
Cc: histonet <@t> lists.utsouthwestern.edu
Betreff: AW: [Histonet] alcohol fixation image (2nd try)

Geoff and Bryan,
Unfortunatly I have no access to these books. But I hoped someone could
describe in a few words the essential differences between formalin and
formalin-fixed tissue.

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754

-----Ursprüngliche Nachricht-----
Von: Geoff McAuliffe [mailto:mcauliff <@t> umdnj.edu] 
Gesendet: Freitag, 17. August 2007 20:46
An: gu.lang <@t> gmx.at
Cc: histonet <@t> lists.utsouthwestern.edu
Betreff: Re: [Histonet] alcohol fixation image (2nd try)

I recall that an early edition (1960's or so) of one of the major 
histology texts (Bloom and Fawcett's, A Textbook of Histology I think) 
had drawing of how and intestinal epithelial cell would look after 
various fixatives. Also try some older editions (pre 1960's) of Maximow 
and Bloom, Bailey, Ham etc, all of the major histo texts that are too 
long and encyclopedic for today's medical student.

Geoff


Gudrun Lang wrote:
> Hi,
>
> I have to admit, that I need help with basic histotechnical knowledge.
> Please, can someone describe me the "alcohol fixation image" of tissue,
> especially chromatin? 
>
> And would poorly formalin-fixed tissue show less chromatin-structure or
more
> intense chromatin-structure, after the usual VIP-processing.
> (50-70-96-96-100-100 alc.) due to the ethanol-effect?
>
>  
>
> Thanks in advance
>
> Gudrun Lang
>
>
>
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> Histonet <@t> lists.utsouthwestern.edu
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>
>   


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
**********************************************


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