[Histonet] Mi Embedding Matrix

[Snider, Vivian Deanna]

Hello netters,

I am in need of your input once again!  

The researchers here use a product called MI Embedding Matrix,
manufactured by Thermo Shandon, (or whatever name they use now) or have
knowledge of it. The researchers here embed their own frozens and I cut
them.  Peridoically over the years we see "holes" where the nuclei
should be. I have contacted Thermo Shandon for their tech support, and
followed everything they suggested. I am at a loss.

 

The tissue being embedded is in vitro skin substitute with human cells;
very small and very thin, so I don't think this is a big issue. 

I cut at 4 - 5 microns at -20.  The only thing affected is the nucleus.
I am really puzzled and do not really know where to turn for answers.
Any thoughts are appreciated.

 

Thanks in advance,

Deanna Snider  HT ASCP

Lead Technologist

Shriners Hospital for Children



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