[Histonet] Mi Embedding Matrix

Snider, Vivian Deanna vsnider <@t> shrinenet.org
Tue Aug 14 10:17:18 CDT 2007

Hello netters,

I am in need of your input once again!  

The researchers here use a product called MI Embedding Matrix,
manufactured by Thermo Shandon, (or whatever name they use now) or have
knowledge of it. The researchers here embed their own frozens and I cut
them.  Peridoically over the years we see "holes" where the nuclei
should be. I have contacted Thermo Shandon for their tech support, and
followed everything they suggested. I am at a loss.


The tissue being embedded is in vitro skin substitute with human cells;
very small and very thin, so I don't think this is a big issue. 

I cut at 4 - 5 microns at -20.  The only thing affected is the nucleus.
I am really puzzled and do not really know where to turn for answers.
Any thoughts are appreciated.


Thanks in advance,

Deanna Snider  HT ASCP

Lead Technologist

Shriners Hospital for Children

CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300.

More information about the Histonet mailing list