[Histonet] Slide Staining Variability

[John Kiernan]

Into trouble with whom? The suppliers of hydrophobic (= greasy) slides that won't work in an automated immunostaining system? Non-wettable (is that a word?) glass slides have no place in biological microtechnique. Many years ago, the cheapest slides on the market had to be washed with detergent and carefully rinsed and dried (distilled water, acetone etc)  before they would accept frozen, paraffin or semithin plastic sections. This was an occasional annoyance in the 1960s. 
 
Since the late 1960s cheap slides have been clean: free of grit and grease. That's my experience. Are there vendors of still-available greasy, dirty, gritty microscope slides? Who buys them?

John Kiernan
London, Canada
------------------

----- Original Message -----
From: "Tarango, Mark" <mtarango <@t> nvcancer.org>
Date: Thursday, August 9, 2007 20:19
Subject: RE: [Histonet] Slide Staining Variability
To: Annette Hall <Annette_hall <@t> pa-ucl.com>, Histonet <@t> lists.utsouthwestern.edu

> I have had this problem before, using the same immunostainer 
> that you're
> using.  The problem was the slides, believe it or 
> not.  The slides we
> were using before were too hydrophobic, aqueous-based reagents would
> bead up and often run right off the slide, into the waste. 
> 
> The slides would work great at keeping the tissue on, but you 
> have to
> make sure that the reagent doesn't run off the slide.  
> There isn't an
> automated stainer that I've seen that can check this.  You 
> might want to
> try different slides and see if it makes a difference. 
> 
> I could tell you which slides weren't working for me, but I 
> don't want
> to get myself into trouble.
> 
> 
> 
> Mark Adam Tarango HT(ASCP)
> 
> Histology/Immunohistochemistry Supervisor
> 
> Nevada Cancer Institute
> 
> One Breakthrough Way
> 
> Las Vegas, NV  89135
> 
> mtarango <@t> nvcancer.org
> 
> Direct Line (702) 822-5112
> 
> Fax (702) 939-7663
> 
>   
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
> AnnetteHall
> Sent: Thursday, August 09, 2007 6:42 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Slide Staining Variability
> 
> Histonetters,
> 
> For those of you who use automated IHC staining systems, has 
> anyone had
> issues with part of the slide staining and the rest of the slide 
> not? We
> have adopted the policy of placing the control on with the patient
> tissue as
> a more comprehensive check of the system. However, we 
> occasionally find
> the
> control is acceptable but the patient fails to even 
> counterstain. This
> has
> been an intermittent problem on the Ventana Benchmark for some 
> time, but
> the
> frequency has been increasing to almost daily.
> 
> Any thoughts?
> 
> Thanks,
> Annette J Hall
> Histo/Cyto/Micro Supervisor
> United Clinical Laboratories
> Dubuque, IA 52001
> 
> -----Original Message-----
> From: Harrison, Sandra C. [mailto:Sandra.Harrison3 <@t> va.gov] 
> Sent: Monday, August 06, 2007 3:35 PM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Leica autostainer w/integrated coverslipper
> 
> Histonetters,
> Could anyone tell me if they're using the Leica AutoStainer XL 
> (ST5010)with the integrated coverslipper (CV5030)?
> Also, can you tell me if you've experienced any trouble with it 
> and how
> long you've been using it?
> 
> Thanks,
> Sandy
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> "EMF <nvcancer.org>" made the following annotations.
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