[Histonet] fixation-question

[Gudrun Lang]

Hi to all fixation-experts,

I've found in the literature some controversy statements about
formaldehyd-fixation and want your help, what is the up-do-date-knowledge.

-          Formaldehyd can/cannot be washed out of fixed tissue.

-          Formaldehyd fixates slower in more acid pH; uncharched
aminogroups react better than protonated with formaldehyd. And these groups
should be uncharged in neutral pH.

-          Nuclear chromatin is fixed only by acid fixatives.

 

What is your opinion on these statements?

 

Regards

Gudrun Lang