[Histonet] acid alcohol with immunos

[Jennifer Cresor]

Hello all,
I have been told in the past to NOT use acid alcohol to differentiate
the hematoxylin on immuno stains and only to use a progressive
hematoxylin staining method. I do not remember the reason why. Do any of
you have experiences and opinions on this subject?  Is there a chance to
harm immuno stains using acid alcohol and if so why? Thank you for your
time.
 
Jennifer
Longview, Wa.
jcresor <@t> lcpath.com