[Histonet] AFB Contamination

Marybeth Kaulahao marybeth.kaulahao <@t> thibodaux.com
Sat Apr 28 17:56:24 CDT 2007

We have been getting false positives as well. The pathologists have decided 
it is the water. Now we use distilled water in the float bath and to rinse 
the slides.
It seems to work so far.
----- Original Message ----- 
From: <Tracey.Lenek <@t> CLS.ab.ca>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, April 24, 2007 3:13 PM
Subject: [Histonet] AFB Contamination


We are experiencing transient false positive ZN staining on skin biopies. 
Today a slide had clumped AFB over the majority of the slide where no tissue 
was present.  Previous to this, only one or two bacilli were noted on the 
Has anyone investigated and found a contaminated waterbath or perhaps the 
Kinyoun's is the culprit as our  lab reuses both?  Any help would be 


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