[Histonet] how to keep toenail on the slide

Joe Nocito jnocito <@t> satx.rr.com
Thu Apr 26 19:36:47 CDT 2007

ouch, get off my toes Kari.
We used to use Nair also, but it was taking too long and I just got down 
right tired of hearing the pathologists complain that their cases were 
taking too long.

----- Original Message ----- 
From: <Histosprv06 <@t> aol.com>
To: <Histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, April 25, 2007 5:49 PM
Subject: Re: [Histonet] how to keep toenail on the slide

> Don't want to step on Joe the Toe's toes (hee hee, ok that was bad)...but 
> I
> have a different way of cutting toenails that seems to work for us. In our 
> lab
> we face into the processed block, turn it upside down and put Nair (yes, 
> the
> hair removal cream) on it for about 3-4 hours (a decent dab). I didn't 
> come
> up  with this, just "inherited" the suggestion and tweeked it a bit. We 
> then
> wipe it off, spray it with Cytocool and cut it (it's so nice and soft by 
> then)
> and put it on a plus slide. We leave really hard nails faced up overnight 
> to
> dry  and then reapply the Nair as necessary until it is soft enough. We 
> then
> "bake"  them in the oven for about 30-40 minutes and let them completely 
> cool
> before  staining. We do PAS-F's on the automated stainer this way as well.
> Our pathologist has not had any problems reading them at all and our 
> slides
> come out beautiful!
> Good luck.
> Kari Zajic, HT,MLT :)
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